Use of supercritical CO2 and ultrasound-assisted extractions to obtain alpha/beta- amyrin-rich extracts from uvaia leaves (Eugenia pyriformis Cambess.)

Author(s)

Klein, Elissandro Jair ^{[1, 2]} ; Santos, Katia Andressa ^[1] ; Palu, Fernando ^[1] ; Adeodato Vieira, Melissa Gurgel ^[2] ; da Silva, Edson Antonio ^[1]

Affiliation

^[1] Western Parana State Univ, Ctr Engn & Exact Sci, Rua Fac 645, BR-85903000 Toledo, PR - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Sch Chem Engn, Ave Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 137, p. 1-8, JUL 2018.
• Web of Science Citations: 7

Abstract

Uvaia (Eugenia pyriformis Cambess.) leaves were subjected to extraction with supercritical fluid extraction (SFE) and ultrasound-assisted extraction (UAE) under different conditions and the results were compared with a conventional maceration extraction. The extracts obtained with SFE were chemically characterized by gas chromatography-mass spectrometry (GC-MS), which showed that beta-amyrin (53.72%) and a-amyrin (24.63%) were their main compounds. The best results for SFE and UAE yieldings were 1.69% and 1.79%, respectively. The best results for amyrin content, by extract weight, were 81.19% for SFE and 77.03% for UAE. The highest amyrin content was 11.40 g of the isomers mixture per kg of dry leaf in SFE and 11.46 g kg(-1) in UAE. Scanning electron microscopy analysis showed differences in leaf particles before and after the extraction process, caused by the removal of compounds by the extraction techniques studied. (AU)

• FAPESP's process: 15/22952-9 - Obtaining and purification of ± and ß-amyrin from leaves Uvaia (Eugenia pyriformis Cambess)
• Grantee: Elissandro Jair Klein
• Support Opportunities: Scholarships in Brazil - Doctorate