Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Features of the relationship between Tomato severe rugose begomovirus and Bemisa tabaci MEAM1 reveal that the virus is acquired during a probe lasting only one minute

Full text
Author(s):
Toloy, Rodrigo Solci [1, 2] ; Mituti, Tatiana [1] ; Sansini Freitas, Debora Maria [3] ; Prado Maluta, Nathalie Kristine [4] ; Zocca Silva, Talita Nicola [1] ; Spotti Lopes, Joo Roberto [4] ; Fereres, Alberto [5] ; Marques Rezende, Jorge Alberto [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Plant Pathol & Nematol, POB 09, BR-13418900 Sao Paulo - Brazil
[2] DuPont Pioneer, POB 3020, BR-77500000 Palmas, Tocantins - Brazil
[3] EMBRAPA Semiarido, POB 23, BR-56302970 Petrolina, Pernambuco - Brazil
[4] Univ Sao Paulo, Dept Entomol & Acarol, POB 09, BR-13418900 Sao Paulo - Brazil
[5] CSIC, Inst Ciencias Agr, E-28006 Madrid - Spain
Total Affiliations: 5
Document type: Journal article
Source: European Journal of Plant Pathology; v. 151, n. 2, p. 541-547, JUN 2018.
Web of Science Citations: 4
Abstract

Tomato severe rugose virus (ToSRV) is a begomovirus found only in Brazil and has been the prevalent species affecting tomato crops in southwest and midwest of the country. Although the virus is known to be transmitted by Bemisia tabaci MEAM1, this study was undertaken to better characterize the relationship between ToSRV and whiteflies on tomato plants. The minimum acquisition and inoculation access periods, latent period, and virus retention time in the vector were determined. The results of transmission experiments showed that the minimum acquisition and inoculation access periods (AAP and IAP) for transmission of ToSRV by MEAM1 were 1 min and 5 min, respectively. After monitoring 50 individual adult whiteflies during a one-minute AAP on ToSRV-infected tomato leaves, 21 insects (42%) were able to acquire the virus, as confirmed by PCR. In contrast, one hundred insects that were individually allowed one-minute AAP on Tomato chlorosis virus (ToCV)-infected tomato leaves, which is a phloem-limited crinivirus, were unable to acquire the virus. The latent period of ToSRV in MEAM1 was 12-15 h, with adult whiteflies retaining the virus for 25 days. Although the tissue distribution of ToSRV in tomato has not been determined, the short AAP suggests that the virus may be present in mesophyll cells, from where it may be acquired during a one-minute feeding probe. (AU)

FAPESP's process: 12/51771-4 - Begomovirus and Crinivirus in Solanaceae: molecular epidemiology and management strategies
Grantee:Jorge Alberto Marques Rezende
Support Opportunities: Research Projects - Thematic Grants