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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stingless Bee Larvae Require Fungal Steroid to Pupate

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Author(s):
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Paludo, Camila R. [1] ; Menezes, Cristiano [2, 3] ; Silva-Junior, Eduardo A. [1] ; Vollet-Neto, Ayrton [2] ; Andrade-Dominguez, Andres [4] ; Pishchany, Gleb [4] ; Khadempour, Lily [5, 6] ; do Nascimento, Fabio S. [2] ; Currie, Cameron R. [5] ; Kolter, Roberto [4] ; Clardy, Jon [7] ; Pupo, Monica T. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Biol, FFCLRP, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Embrapa Amazonia Oriental, Brazilian Agr Res Corp, BR-66095100 Belem, Para - Brazil
[4] Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 - USA
[5] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 - USA
[6] Univ Wisconsin, Dept Zool, Madison, WI 53706 - USA
[7] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 - USA
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, JAN 18 2018.
Web of Science Citations: 9
Abstract

The larval stage of the stingless bee Scaptotrigona depilis must consume a specific brood cell fungus in order to continue development. Here we show that this fungus is a member of the genus Zygosaccharomyces and provides essential steroid precursors to the developing bee. Insect pupation requires ecdysteroid hormones, and as insects cannot synthesize sterols de novo, they must obtain steroids in their diet. Larval in vitro culturing assays demonstrated that consuming ergosterol recapitulates the developmental effects on S. depilis as ingestion of Zygosaccharomyces sp. cells. Thus, we determined the molecular underpinning of this intimate mutualistic symbiosis. Phylogenetic analyses showed that similar cases of bee-Zygosaccharomyces symbiosis may exist. This unprecedented case of bee-fungus symbiosis driven by steroid requirement brings new perspectives regarding pollinator-microbiota interaction and preservation. (AU)

FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 13/50954-0 - Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates
Grantee:Mônica Tallarico Pupo
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 12/24204-1 - Natural products involved in chemical and biological interactions of microorganisms associated to nests of the leaf-cutting ants Atta Sexdens rubropilosa
Grantee:Eduardo Afonso da Silva Junior
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/04092-7 - Natural products from microorganisms associated with the social insects Scaptotrigona depilis and Atta sexdens rubropilosa: chemical characterization, biological and ecological functions
Grantee:Mônica Tallarico Pupo
Support type: Regular Research Grants
FAPESP's process: 12/22487-6 - Chemical and biological study of natural products of microorganisms associated with stingless bee species Scaptotrigona depilis
Grantee:Camila Raquel Paludo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/23532-0 - Genetic sequencing, ecological and chemical aspects of Bacillus SP. isolated from the larval stage of stingless bee Scaptotrigona depilis
Grantee:Camila Raquel Paludo
Support type: Scholarships abroad - Research Internship - Doctorate