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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Full text
Author(s):
Costa Barreira, Luzia Aparecida [1] ; Scheucher, Priscila Santos [2] ; Romeiro, Marilia Farignoli [1] ; La Serra, Leonardo [1] ; Badra, Soraya Jabur [1] ; de Souza, William Marciel [1] ; Moraes Figueiredo, Luiz Tadeu [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Pesquisa Virol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Hematol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 51, n. 2, p. 168-173, MAR-APR 2018.
Web of Science Citations: 1
Abstract

Abstract INTRODUCTION: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. METHODS: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. CONCLUSIONS: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories. (AU)

FAPESP's process: 17/13981-0 - Characterization, genomics and diagnostic of viruses with importance for public health in Brazil by high throughput sequencing
Grantee:William Marciel de Souza
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
Grantee:William Marciel de Souza
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/02438-6 - Studies with Bunyaviridae that produce human disease
Grantee:Luiz Tadeu Moraes Figueiredo
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
Grantee:Marilia Farignoli Romeiro
Support Opportunities: Scholarships in Brazil - Doctorate