Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Full text
Costa Barreira, Luzia Aparecida [1] ; Scheucher, Priscila Santos [2] ; Romeiro, Marilia Farignoli [1] ; La Serra, Leonardo [1] ; Badra, Soraya Jabur [1] ; de Souza, William Marciel [1] ; Moraes Figueiredo, Luiz Tadeu [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Pesquisa Virol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Hematol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 51, n. 2, p. 168-173, MAR-APR 2018.
Web of Science Citations: 1

Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: in this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerise chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories. (AU)

FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/13981-0 - Characterization, genomics and diagnostic of viruses with importance for public health in Brazil by high throughput sequencing
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/02438-6 - Studies with Bunyaviridae that produce human disease
Grantee:Luiz Tadeu Moraes Figueiredo
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
Grantee:Marilia Farignoli Romeiro
Support type: Scholarships in Brazil - Doctorate