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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Signaling pathways involved in zymosan phagocytosis induced by two secreted phospholipases A(2) isolated from Bothrops asper snake venom in macrophages

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Author(s):
Zuliani, Juliana Pavan [1, 2, 3] ; Maria Gutierrez, Jose [4] ; Teixeira, Catarina [2]
Total Authors: 3
Affiliation:
[1] Univ Fed Rondonia, UNIR, Dept Med, Porto Velho, RO - Brazil
[2] Inst Butantan, Lab Farmacol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
[3] Fundacao Oswaldo Cruz Rondonia FIOCRUZ RO, Lab Imunol Celular Aplicada Saude, Porto Velho, RO - Brazil
[4] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose - Costa Rica
Total Affiliations: 4
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 113, p. 575-582, JUL 1 2018.
Web of Science Citations: 2
Abstract

Phagocytosis, a process involved in host defense, requires coordination of a variety of signaling reactions. MT-II, a catalytically-inactive Lys49-PLA(2), and MT-III, an active Asp49-PLA(2) isolated from Bothrops asper snake venom, activate phagocytosis in macrophages. In this study the signal pathways mediating zymosan phagocytosis, focusing in lipidic second messengers, were investigated. Macrophages collected from male Swiss mouse peritoneum were obtained 96 h after i.p. injection of thioglycollate. Phagocytosis was evaluated with non-opsonized zymosan in the presence or absence of specific inhibitors. Data showed that both venom PLA(2)s increased phagocytosis. Zileuton, Etoricoxib, PACOCF3 (5-LO, COX-2 and iPLA(2) inhibitors, respectively), as well as WEB2170 (PAF receptor antagonist) significantly reduced phagocytosis induced by both venom PLA(2)s. However, Indomethacin (COX-1/COX-2 inhibitor) and Montelukast (CysL receptor antagonist) did not affect the toxins-induced phagocytosis. Moreover, while PACOCF3 (iPLA(2) inhibitor), reduced the phagocytosis induced by MT-II and MT-III, AACOCF3 (cPLA(2) inhibitor) significantly reduced the MT-II, but not MT-Ill-induced phagocytosis. These data suggest the effect of both sPLA(2)s depends on IPLA(2) and that the effect of MT-II depends on activation of cPLA(2). COX-2 and 5-W-derived metabolites as well as PAF are involved in the signaling events required for phagocytosis induced by both venom sPLA(2)s. (C) 2018 Elsevier B.V. All rights reserved. (AU)