| Full text | |
| Author(s): |
Cardoso, Sara
[1, 2]
;
Sousa, Angela
[1]
;
Queiroz, Joao A.
[1]
;
Azzoni, Adriano R.
[2]
;
Sousa, Fani
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Beira Interior, CICS UBI Hlth Sci Res Ctr, Av Infante 10 Henrique, P-6201506 Covilha - Portugal
[2] Univ Sao Paulo, Dept Engn Quim, Escola Politecn, Av Prof Linea Prestes, 580 Bloco 20, BR-05424970 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1087, p. 149-157, JUN 15 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Purification of plasmid DNA targeting therapeutic applications still presents many challenges, namely on supports and specific ligand development. Monolithic supports have emerged as interesting approaches for purifying pDNA due to its excellent mass transfer properties and higher binding capacity values. Moreover, arginine ligands were already described to establish specific and preferential interactions with pDNA. Additionally, some studies revealed the ability of arginine based cationic peptides to condense plasmid DNA, which increased lengthening can result in strongest interactions with higher binding capacities for chromatographic purposes of large molecules such as pDNA. In this work, arginine homopeptides were immobilized in monolithic supports and their performance was evaluated and compared with a single arginine monolithic column regarding supercoiled (sc) plasmid DNA purification. Specific interactions of arginine based peptides with several nucleic acids present in a clarified Escherichia coli lysate sample showed potential for the sc pDNA purification. Effectively, the immobilization of the arginine homopeptides became more functional compared with the single arginine amino acid, showing higher binding capacities, which was also reflected in the intensity of the interactions. The combination of structural versatilities of monoliths with the specificity of arginine peptides raised as a promising strategy for sc pDNA purification. (AU) | |
| FAPESP's process: | 13/23780-1 - Development of non-viral gene delivery vectors based on the Rp3 Dynein light chain |
| Grantee: | Adriano Rodrigues Azzoni |
| Support Opportunities: | Regular Research Grants |