Cellular response of pulp fibroblast to single or multiple photobiomodulation applications

Author(s)

Fernandes, Amanda ^[1] ; Lourenco Neto, Natalino ^[1] ; Teixeira Marques, Nadia Carolina ^[2] ; Ribeiro Vitor, Luciana Lourenco ^[1] ; Oliveira Prado, Mariel Tavares ^[1] ; Oliveira, Rodrigo Cardoso ^[3] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Oliveira, Thais Marchini ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Jose do Rosario Vellano Univ, UNIFENAS, Dept Pediat Dent, Rodovia MG 179, Km 0, BR-37130000 Alfenas, MG - Brazil

^[3] Univ Sao Paulo Alameda, Bauru Sch Dent, Dept Biol Sci, Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: Laser Physics; v. 28, n. 6 JUN 2018.
• Web of Science Citations: 1

Abstract

This study aimed to evaluate in vitro the effects of single or multiple photobiomodulation (PBM) applications on the viability and proliferation of pulp fibroblasts. Pulp fibroblasts from human deciduous teeth were obtained from a biorepository, plated into 96-well plates, and irradiated according to the experimental groups. At 24 h, 48 h, and 72 h after irradiation, cell viability and proliferation were assessed through MTT and Crystal Violet assays, respectively. The intragroup comparison revealed statistically significant differences for 2.5 J cm(-2) (3x) with increasing viability at 72 h over 48 h (p = 0.027). The intergroup analysis showed a greater viability of the multiple PBM applications 2.5 J cm(-2) (3x) over the single application 7.5 J cm(-2) (1x) at 72 h. The application of 5 J cm(-2) (1x) exhibited greater proliferation than the application of 7.5 J cm(-2) (1x), 2.5 J cm(-2) (2x) and 2.5 J cm(-2) (3x). Single or multiple PBM applications demonstration different stimulatory effects on pulp fibroblast. The results show that the group submitted to multiple irradiation presented significantly higher cell viability than the groups with single irradiation at 72 h. However, the photobiomodulation therapy with single irradiations was more effective on cell proliferation at 24 h. (AU)

• FAPESP's process: 13/18886-5 - Low intensity laser effects of different energy densities in cells of the pulp of human deciduous teeth
• Grantee: Thais Marchini de Oliveira Valarelli
• Support Opportunities: Regular Research Grants