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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms

Full text
Author(s):
Pamela Oliveira de Souza de Azevedo [1] ; Francesco Molinari [2] ; Ricardo Pinheiro de Souza Oliveira [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil
[2] Univ Milan, Dept Food Environm & Nutr Sci DeFENS, Sect Food Microbiol & Bioproc, Milan - Italy
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 54, n. 1 2018-06-07.
Abstract

Abstract Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response. (AU)

FAPESP's process: 16/06284-9 - Biotechnology production of pediocin: optimization of yield and biological effects on cells of the immune system
Grantee:Ricardo Pinheiro de Souza Oliveira
Support Opportunities: Regular Research Grants