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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cutaneous fungal microbiome: Malassezia yeasts in seborrheic dermatitis scalp in a randomized, comparative and therapeutic trial

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Author(s):
Kamamoto, C. S. L. [1] ; Nishikaku, A. S. [2] ; Gompertz, O. F. [3] ; Melo, A. S. [2] ; Hassun, K. M. [1] ; Bagatin, E. [1]
Total Authors: 6
Affiliation:
[1] Fed Univ Sao Paulo UNIFESP, Dept Dermatol, Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Med, Special Mycol Lab, Sao Paulo, SP - Brazil
[3] Fed Univ Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: DERMATO-ENDOCRINOLOGY; v. 9, n. 1 OCT 23 2017.
Web of Science Citations: 3
Abstract

Malassezia spp in skin microbiome scalp has been implicated in seborrheic dermatitis pathogenesis. Thus, treatment based in antifungal combined to topical keratolitic agents have been indicated as well as oral isotretinoin as it reduces the sebum production, glandular's size and possesses antiinflammatory properties. This randomized, comparative and therapeutic trial aimed toper form the genotypic identification of Malassezia species before and after low-dose oral isotretinoin or topical antifungal treatments for moderate to severe seborrhea and/or seborrheic dermatitis on scalp. Scales and sebum of the scalp were seeded in the middle of modified Dixon and incubated at 32 degrees C. For genotypic identification polymerase chain reaction primers for the ITS and D1/D2 ribossomal DNA were used and followed by samples sequencing. The procedure was conducted before and after therapeutic and randomized intervention for moderate to severe seborrhea/seborrheic dermatitis on the scalp, including oral isotretinoin, 10 mg, every other day and anti-seborrheic shampoo (piroctone olamine), over six months. The M. globosa and M. restricta were the most frequent species isolated on the scalp before and after both treatments. Other non-Malassezia species were also identified. The Malassezia spp. were maintained in the scalp after both treatments that were equally effective for the control of seborrhea/seborrheic dermatitis clinical signs. (AU)

FAPESP's process: 10/51049-1 - Clinical, laboratorial and quality of life trial to evaluate the efficacy and safety of low-dose oral isotretinoin for seborrhea
Grantee:Ediléia Bagatin
Support Opportunities: Regular Research Grants