&ITShort communication&IT:The first report of &ITCyberlindnera rhodanensis&IT associated with clinical bovine mastitis

Author(s)

Dalanezi, Felipe Morales ^[1] ; da Paz, Giselle Souza ^[1] ; Joaquim, Samea Fernandes ^[1] ; Guimaraes, Felipe Freitas ^[1] ; Gimenes Bosco, Sandra de Moraes ^[2] ; Langoni, Helio ^[1]

Affiliation

^[1] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, BR-18618681 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Microbiol & Immunol, BR-18618681 Botucatu, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF DAIRY SCIENCE; v. 101, n. 1, p. 581-583, JAN 2018.
• Web of Science Citations: 0

Abstract

An acute case of clinical mastitis in a Holstein cow from second lactation is reported here. A milk sample from the affected quarter was cultured on 5% bovine blood agar and incubated at 37 degrees C for 72 h. After 24 h of incubation, numerous colonies of yeast were observed: the Candida characteristic was not detected by CHROMagar Candida (Difco, Franklin Lakes, NJ). The DNA extraction of the isolate was performed, and DNA. was subjected to amplification and sequencing of the D1/D2 region of the large subunit rRNA gene. The sequences were aligned using Mega 7.0 and used for searching GenBank by BLASTn (Basic Local Alignment Search Tool for nucleotides), revealing 98% of identity with Cyberlindnera rhodanensis. To date, this is the first report of this yeast associated with clinical bovine mastitis. (AU)

• FAPESP's process: 15/26055-1 - Influence of pathogens causing clinical mastitis in dairy cows reproductive indices
• Grantee: Felipe Morales Dalanezi
• Support Opportunities: Scholarships in Brazil - Doctorate