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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops

Full text
Author(s):
Giovanna V. Guidelli [1] ; Lucia Mattiello [2] ; Rafael H. Gallinari [3] ; Paulo Cezar de Lucca [4] ; Marcelo Menossi [5]
Total Authors: 5
Affiliation:
[1] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética - Brasil
[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética - Brasil
[3] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética - Brasil
[4] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução, Microbiologia e Imunologia - Brasil
[5] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética - Brasil
Total Affiliations: 5
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 41, n. 2, p. 450-454, 2018-06-11.
Abstract

Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene from pGVG showed high levels of GUS activity. qRT-PCR evaluations demonstrated success for both overexpression and hairpin-based silencing cassettes. Therefore, pGVG is suitable for plant transformation and subsequent applications for high-throughput production of stable transgenic sugarcane. The use of an expression cassette based on the ZmUbi1 promoter opens the possibility of using pGVG in other monocot species. (AU)

FAPESP's process: 14/26521-0 - Going deeper into drought tolerance in sugarcane: molecular analysis of root responses
Grantee:Giovanna Vieira Guidelli
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/08659-7 - Development of a methodology to produce genetically modified plants to be applied in basic genetics and plant breeding
Grantee:Paulo Cezar de Lucca
Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
FAPESP's process: 13/15576-5 - Molecular approaches to study the relationship between ethylene and sugarcane ripening
Grantee:Marcelo Menossi Teixeira
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 15/23789-4 - EVALUATION OF THE ROLE OF GENES ENCODING ACC SINTHASE IN THE DEVELOPMENT OF SUGARCANE PLANTS
Grantee:Lucia Mattiello
Support Opportunities: Scholarships in Brazil - Post-Doctoral