| Full text | |
| Author(s): |
Santos, Daubian
[1]
;
Ribeiro, Guilherme Cunha
[1]
;
Cabral, Aline Diniz
[1]
;
Speranca, Marcia Aparecida
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PLoS One; v. 13, n. 2 FEB 1 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
There is a growing necessity to integrate morphological and genetic studies. This paper proposes a new technique that allows DNA extraction of arthropods while still keeping intact the entire morphology of the specimens. The technique uses Proteinase K to dissolve protein tissues and preserve the chitinous exoskeleton of specimens. The method is fast, cheap, non-toxic, and allows for good morphological preparations of specimens retaining much of their tridimensional structure. The methodology works fine with specimens preserved in different kinds of media, such as for dry (pinned) specimens, and specimens preserved in Ethanol. In addition, it allows the extraction of DNA from fresh specimens, as well as from specimens preserved for a long time. The technique works well for morphological studies alone, but allows the generation of an associated genomic library at an individual-scale. Among the advantages of the new technique is the possibility of extracting DNA from the entire specimen (necessary for the study of diseases transmitted by arthropod vectors), while still keeping the morphology intact for correct taxonomic identification. In addition, in comparison with methods that extract DNA from small tissue samples (e.g., from legs or wings), the method allows for the extraction of a larger amount of DNA and is better suited for small specimens. (AU) | |
| FAPESP's process: | 16/14514-4 - Characterization of the chitinase from South American endemic Leishmania species: use in diagnosis in humans, dogs and sandflies. |
| Grantee: | Marcia Aparecida Speranca |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/26096-4 - Heterologous expression of the chitinase enzyme of Leishmania (L.) infantum chagasi, Leishmania (V.) braziliensis and Leishmania (V.) amazonensis: serological diagnosis and comparative molecular study using expression systems of insects cell and bacteria |
| Grantee: | Aline Diniz Cabral |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |