| Full text | |
| Author(s): |
Moretti, Nilmar Silvio
[1, 2]
;
Cestari, Igor
[1]
;
Anupama, Atashi
[1]
;
Stuart, Ken
[1]
;
Schenkman, Sergio
[2]
Total Authors: 5
|
| Affiliation: | [1] Ctr Infect Dis Res, 307 Westlake Ave North, Suite 500, Seattle, WA 98109 - USA
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, R Pedro de Toledo 669 L6A, BR-04039032 Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF PROTEOME RESEARCH; v. 17, n. 1, p. 374-385, JAN 2018. |
| Web of Science Citations: | 4 |
| Abstract | |
Protein acetylation is a post-translational modification regulating diverse cellular processes. By using proteomic approaches, we identified N-terminal and epsilon-lysine acetylated proteins in Trypanosoma cruzi and Trypanosoma brucei, which are protozoan parasites that cause significant human and animal diseases. We detected 288 lysine acetylation sites in 210 proteins of procyclic form, an insect stage of T. brucei, and 380 acetylation sites in 285 proteins in the form of the parasite that replicates in mammalian bloodstream. In T. cruzi insect proliferative form we found 389 epsilon-lysine-acetylated sites in 235 proteins. Notably, we found distinct acetylation profiles according to the developmental stage and species, with only 44 common proteins between T. brucei stages and 18 in common between the two species. While K-ac proteins from T. cruzi are enriched in enzymes involved in oxidation/reduction balance, required for the parasite survival in the host, in T. brucei, most K-ac proteins are enriched in metabolic processes, essential for its adaptation in its hosts. We also identified in both parasites a quite variable N-terminal acetylation sites. Our results suggest that protein acetylation is involved in differential regulation of multiple cellular processes in Trypanosomes, contributing to our understanding of the essential mechanisms for parasite infection and survival. (AU) | |
| FAPESP's process: | 11/51973-3 - Cell signaling mechanism of Trypanosoma in response to nutritional alterations and genotoxic agents |
| Grantee: | Sergio Schenkman |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/22031-0 - Cell signaling in Trypanosoma during host-parasite interaction |
| Grantee: | Sergio Schenkman |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/20074-9 - Global analysis of protein acetylation in Trypanosoma cruzi |
| Grantee: | Nilmar Silvio Moretti |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |