Physical exercise reduces pyruvate carboxylase (PCB) and contributes to hyperglycemia reduction in obese mice

Author(s):Show less -

Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; Crisol, Barbara Moreira ^[1] ; Formigari, Guilherme Pedron ^[2] ; Sant'Ana, Marcella Ramos ^[2] ; Botezelli, Jose Diego ^[1] ; Gaspar, Rodrigo Stellzer ^[1] ; da Silva, Adelino S. R. ^{[3, 4]} ; Cintra, Dennys Esper ^{[5, 2]} ; de Moura, Leandro Pereira ^{[1, 5, 6]} ; Ropelle, Eduardo Rochete ^{[1, 5, 6]} ; Pauli, Jose Rodrigo ^{[1, 5, 6]}

Affiliation

^[1] Univ Campinas UNICAMP, Lab Mol Biol Exercise, Limeira, SP - Brazil

^[2] Univ Campinas UNICAMP, Lab Nutr Genom, Limeira, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeiro Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Campinas UNICAMP, OCRC Obes & Comorbid Res Ctr, Campinas, SP - Brazil

^[6] Univ Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP - Brazil

• Document type: Journal article
• Source: Journal of Physiological Sciences; v. 68, n. 4, p. 493-501, JUL 2018.
• Web of Science Citations: 3

Abstract

The present study evaluated the effects of exercise training on pyruvate carboxylase protein (PCB) levels in hepatic tissue and glucose homeostasis control in obese mice. Swiss mice were distributed into three groups: control mice (CTL), fed a standard rodent chow; diet-induced obesity (DIO), fed an obesity-inducing diet; and a third group, which also received an obesity-inducing diet, but was subjected to an exercise training protocol (DIO + EXE). Protocol training was carried out for 1 h/d, 5 d/wk, for 8 weeks, performed at an intensity of 60% of exhaustion velocity. An insulin tolerance test (ITT) was performed in the last experimental week. Twenty-four hours after the last physical exercise session, the animals were euthanized and the liver was harvested for molecular analysis. Firstly, DIO mice showed increased epididymal fat and serum glucose and these results were accompanied by increased PCB and decreased p-Akt in hepatic tissue. On the other hand, physical exercise was able to increase the performance of the mice and attenuate PCB levels and hyperglycemia in DIO + EXE mice. The above findings show that physical exercise seems to be able to regulate hyperglycemia in obese mice, suggesting the participation of PCB, which was enhanced in the obese condition and attenuated after a treadmill running protocol. This is the first study to be aimed at the role of exercise training in hepatic PCB levels, which may be a novel mechanism that can collaborate to reduce the development of hyperglycemia and type 2 diabetes in DIO mice. (AU)

• FAPESP's process: 13/21491-2 - Role of physical exercise in regulation of rock protein and the effects on insulin signaling in skeletal muscle and liver in obese mice
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/18488-8 - The role of physical exercise on molecular pathways of glucose uptake in GLUT-4 knockout mice
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants