Immunohistochemical investigation of the cross-reactivity of selected cell markers in formalin-fixed, paraffin-embedded lymphoid tissues of Franciscana (Pontoporia blainvillei)

Author(s)

Diaz-Delgado, J. ^{[1, 2]} ; Ressio, R. ^[2] ; Groch, K. R. ^[1] ; Catao-Dias, J. L. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Av Prof Orlando Marques de Paiva 87, Sao Paulo - Brazil

^[2] IAL, Ctr Patol, Av Dr Arnaldo, 351-7 Andar, Sala 706, Sao Paulo - Brazil

• Document type: Journal article
• Source: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 200, p. 52-58, JUN 2018.
• Web of Science Citations: 2

Abstract

A considerable amount of knowledge on natural and anthropogenic pathologic conditions affecting different cetacean species has been gained over the last decades. Nonetheless, the immunopathological bases for most of these processes have been poorly documented or remain unknown. Comparative immunopathological investigations in these species are precluded by the limited number of specific antibodies, most of which are not commercially available, and the reduced spectrum of validated and/or cross-reactive ones. To partially fill in this gap of knowledge, a set of commercially available primary antibodies were tested for cross-reactivity against leukocytes and cytokines in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissues (lymph nodes, spleen and thymus) of three bycaught, apparently healthy and fresh Franciscanas (Pontoporia blainvillei) using immunohistochemistry. On the basis of similar region specificity within the lymphoid organs, cellular morphology and staining pattern with human control tissues, 13/19 primary antibodies (caspase 3, CD3, CD57, CD68, FoxP3, FILA-DR alpha, IFN gamma, IgG, IL4, IL10, Lysozyme, TGF beta and PAX-5) exhibited satisfactory cross-reactivity. Our results expand the spectrum of suitable cross-reactive primary antibodies in FFPE cetacean tissues. Further comparative immunopathological studies focused on infectious diseases and ecotoxicology may benefit from establishment of baseline expression of immunologically relevant molecules in various cetaceans species. (AU)

• FAPESP's process: 14/24932-2 - Research and characterization of Morbiliviruses of cetaceans in Brazil
• Grantee: Kátia Regina Groch
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/00735-6 - Research and characterization of Morbiliviruses of cetaceans in Brazil
• Grantee: Jose Luiz Catao Dias
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/02223-8 - Comparative immunopathology of cetacean morbillivirus infection: histologic, immunohistochemical, and molecular studies
• Grantee: Josué Díaz Delgado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral