Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches

Full text
Author(s):
Sporck, Daniele [1] ; Reinoso, Felipe A. M. [1] ; Rencoret, Jorge [2] ; Gutierrez, Ana [2] ; del Rio, Jose C. [2] ; Ferraz, Andre [1] ; Milagres, Adriane M. F. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, BR-12602810 Lorena, SP - Brazil
[2] CSIC, Inst Recursos Nat & Agrobiol Sevilla, Av Reina Mercedes 10, Seville 41012 - Spain
Total Affiliations: 2
Document type: Journal article
Source: BIOTECHNOLOGY FOR BIOFUELS; v. 10, DEC 7 2017.
Web of Science Citations: 11
Abstract

Background: New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis of the glucan component is a way to add value to the hemicellulose fraction, which can be used in papermaking, pharmaceutical, and food industries. Hemicellulose removal can also facilitate subsequent cellulolytic glucan hydrolysis. Results: Sugarcane bagasse was pretreated with an alkaline-sulfite chemithermomechanical process to facilitate subsequent extraction of xylan by enzymatic or alkaline procedures. Alkaline extraction methods yielded 53% (w/w) xylan recovery. The enzymatic approach provided a limited yield of 22% (w/w) but produced the xylan with the lowest contamination with lignin and glucan components. All extracted xylans presented arabinosyl side groups and absence of acetylation. 2D-NMR data suggested the presence of O-methyl-glucuronic acid and p-coumarates only in enzymatically extracted xylan. Xylans isolated using the enzymatic approach resulted in products with molecular weights (Mw) lower than 6 kDa. Higher Mw values were detected in the alkali-isolated xylans. Alkaline extraction of xylan provided a glucan-enriched solid readily hydrolysable with low cellulase loads, generating hydrolysates with a high glucose/xylose ratio. Conclusions: Hemicellulose removal before enzymatic hydrolysis of the cellulosic fraction proved to be an efficient manner to add value to sugarcane bagasse biorefining. Xylans with varied yield, purity, and structure can be obtained according to the extraction method. Enzymatic extraction procedures produce high-purity xylans at low yield, whereas alkaline extraction methods provided higher xylan yields with more lignin and glucan contamination. When xylan extraction is performed with alkaline methods, the residual glucan-enriched solid seems suitable for glucose production employing low cellulase loadings. (AU)

FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 08/56256-5 - Topochemistry, porosity and chemical composition determining successful enzymatic sacarification of sugarcane bagasse
Grantee:Adriane Maria Ferreira Milagres
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants