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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PHBV-TiO2 mats prepared by electrospinning technique: Physico-chemical properties and cytocompatibility

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Braga, Natalia F. [1] ; Vital, Daniel A. [1] ; Guerrini, Lilia M. [1] ; Lemes, Ana P. [1] ; Formaggio, Daniela M. D. [1] ; Tada, Dayane B. [1] ; Arantes, Tatiane M. [2] ; Cristovan, Fernando H. [2]
Total Authors: 8
[1] Unifesp Fed Univ Sao Paulo, Rua Talim 330, BR-12231280 Sao Paulo - Brazil
[2] Fed Univ Jatai, BR 364, Km 195, 3800, BR-75801615 Jatai, Go - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Biopolymers; v. 109, n. 5 MAY 2018.
Web of Science Citations: 5

One of the most important challenges in tissue engineering research is the development of biomimetic materials. In this present study, we have investigated the effect of the titanium dioxide (TiO2) nanoparticles on the properties of electrospun mats of poly (hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), to be used as scaffold. The morphology of electrospun fibers was observed by scanning electron microscopy (SEM). Both pure PHBV and nanocomposites fibers were smooth and uniform. However, there was an increase in fiber diameter with the increase of TiO2 concentration. Thermal properties of PHBV and nanocomposite mats were characterized by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). DSC analysis showed that the crystallization temperature for PHBV shifts to higher temperature in the presence of the nanoparticles, indicating that TiO2 nanoparticles change the process of crystallization of PHBV due to heterogeneous nucleation effect. TGA showed that in the presence of the nanoparticles, the curves are shifted to lower temperatures indicating a decreasing in thermal stability of nanocomposites compared to pure PHBV. To produce scaffolds for tissue engineering, it is important to evaluate the biocompatibility of the material. Cytotoxicity assay showed that TiO2 nanoparticles were not cytotoxic for cells at the concentration used to synthesize the mats. The proliferation of cells on the mats was evaluated by the MTT assay. Results showed that the nanocomposite samples increased cell proliferation compared to the pure PHBV. These results indicate that continuous electrospun fibrous scaffolds may be a good substrate for tissue regeneration. (AU)

FAPESP's process: 11/23895-8 - Characterization and control of cellular internalization process of nanoparticles
Grantee:Dayane Batista Tada
Support type: Research Grants - Young Investigators Grants