Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study

Author(s)

Borges, Aline Chiodi ^{[1, 2]} ; Gouvea Lima, Gabriela de Morais ^{[1, 2]} ; Castaldelli Nishime, Thalita Mayumi ^[3] ; Lacerda Gontijo, Aline Vidal ^{[1, 2]} ; Kostov, Konstantin Georgiev ^[3] ; Koga-Ito, Cristiane Yumi ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Environm Engn, Inst Sci & Technol, Sao Jose Dos Campos - Brazil

^[2] Sao Paulo State Univ UNESP, Oral Biopathol Grad Program, Inst Sci & Technol, Sao Jose Dos Campos - Brazil

^[3] Sao Paulo State Univ UNESP, Guaratingueta Fac Engn, Dept Chem & Phys, Guaratingueta - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 6 JUN 27 2018.
• Web of Science Citations: 3

Abstract

The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 +/- 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24 +/- 15.23% and 88.96 +/- 18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity. (AU)

• FAPESP's process: 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance
• Grantee: Cristiane Yumi Koga Ito
• Support type: Regular Research Grants
• FAPESP's process: 15/21989-6 - Development of Cold Atmospheric Pressure Plasma Jets for Material Treatment, Thin Films Deposition and Biomedical Applications
• Grantee: Konstantin Georgiev Kostov
• Support type: Regular Research Grants
• FAPESP's process: 14/02354-7 - Antifungal activity and genotoxicity of atmospheric pressure nonthermal plasma jet
• Grantee: Aline Chiodi Borges
• Support type: Scholarships in Brazil - Doctorate