| Full text | |
| Author(s): Show less - |
Mallinson, Sam J. B.
[1]
;
Machovina, Melodie M.
[2, 3]
;
Silveira, Rodrigo L.
[4, 3]
;
Garcia-Borras, Marc
[5]
;
Gallup, Nathan
[5, 3]
;
Johnson, Christopher W.
[3]
;
Allen, Mark D.
[1]
;
Skaf, Munir S.
[4]
;
Crowley, Michael F.
[6]
;
Neidle, Ellen L.
[7]
;
Houk, Kendall N.
[5]
;
Beckham, Gregg T.
[3]
;
DuBois, Jennifer L.
[2]
;
McGeehan, John E.
[1]
Total Authors: 14
|
| Affiliation: | [1] Univ Portsmouth, Sch Biol Sci, Inst Biol & Biomed Sci, Mol Biophys, Portsmouth PO1 2DY, Hants - England
[2] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 - USA
[3] Natl Bioenergy Ctr, Natl Renewable Energy Lab, Golden, CO 80401 - USA
[4] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
[5] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 - USA
[6] Biosci Ctr, Natl Renewable Energy Lab, Golden, CO 80401 - USA
[7] Univ Georgia, Dept Microbiol, Athens, GA 30602 - USA
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | NATURE COMMUNICATIONS; v. 9, JUN 27 2018. |
| Web of Science Citations: | 23 |
| Abstract | |
Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion. (AU) | |
| FAPESP's process: | 13/08293-7 - CCES - Center for Computational Engineering and Sciences |
| Grantee: | Munir Salomao Skaf |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 14/10448-1 - Molecular aspects of plant cell wall architecture |
| Grantee: | Rodrigo Leandro Silveira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/22956-7 - Hybrid QM/MM simulations of feruloyl esterases: cleavage mechanism of lignin-carbohydrate complexes in plant cell walls |
| Grantee: | Rodrigo Leandro Silveira |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |