Regulation of extracellular matrix elements and sarcomerogenesis in response to different periods of passive stretching in the soleus muscle of rats

Author(s)

Peviani, Sabrina M. ^[1] ; Guzzoni, Vinicius ^[2] ; Pinheiro-Dardis, Clara M. ^[1] ; da Silva, Yara P. ^[1] ; Fioravante, Alisson C. R. ^[1] ; Sagawa, Adriana H. ^[1] ; Delfino, Gabriel B. ^[1] ; Durigan, Joao L. Q. ^[3] ; Salvini, Tania F. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Phys Therapy, Sao Carlos, SP - Brazil

^[2] Univ Brasilia, Brasilia, DF - Brazil

^[3] Univ Brasilia, Grad Program Rehabil Sci, Brasilia, DF - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, JUN 13 2018.
• Web of Science Citations: 3

Abstract

Stretching is a common method used to prevent muscle shortening and improve limited mobility. However, the effect of different time periods on stretching-induced adaptation of the extracellular matrix and its regulatory elements have yet to be investigated. We aimed to evaluate the expression of fibrillar collagens, sarcomerogenesis, metalloproteinase (MMP) activity and gene expression of the extracellular matrix (ECM) regulators in the soleus (SOL) muscle of rats submitted to different stretching periods. The soleus muscles were submitted to 10 sets of passive stretching over 10 (St 10d) or 15 days (St 15d) (1 min per set, with 30 seconds' rest between sets). Sarcomerogenesis, muscle cross-sectional area (CSA), and MMP activity and mRNA levels in collagen (type I, III and IV), connective tissue growth factor (CTGF), growth factor-beta (TGF-beta), and lysyl oxidase (LOX) were analyzed. Passive stretching over both time periods mitigated COL-I deposition in the SOL muscle of rats. Paradoxically, 10 days of passive stretching induced COL-I and COL-III synthesis, with concomitant upregulation of TGF-beta 1 and CTGF at a transcriptional level. These responses may be associated with lower LOX mRNA levels in SOL muscles submitted to 10 passive stretching sessions. Moreover, sarcomerogenesis was observed after 15 days of stretching, suggesting that stretching-induced muscle adaptations are time-dependent responses. (AU)

• FAPESP's process: 08/03499-8 - The effect of anterior cruciate ligament rupture in morphology, gene and protein expressions related to atrophy in the quadriceps femoral muscle of rats
• Grantee: Gabriel Borges Delfino
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 11/11563-0 - Extracellular matrix and morphology adaptations in rat soleus muscle after passive stretching: implications for rehabilitation
• Grantee: Alisson Ricardo Carvalho Fioravante
• Support Opportunities: Scholarships in Brazil - Scientific Initiation