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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial production of toxins from the scorpion venom: properties and applications

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Author(s):
Amorim, Fernanda Gobbi [1] ; Cordeiro, Francielle Almeida [1] ; Pinheiro-Junior, Ernesto Lopes [1] ; Boldrini-Franca, Johara [1] ; Arantes, Eliane Candiani [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Review article
Source: Applied Microbiology and Biotechnology; v. 102, n. 15, p. 6319-6331, AUG 2018.
Web of Science Citations: 1
Abstract

Scorpion venom are composed mainly of bioactive proteins and peptides that may serve as lead compounds for the design of biotechnological tools and therapeutic drugs. However, exploring the therapeutic potential of scorpion venom components is mainly impaired by the low yield of purified toxins from milked venom. Therefore, production of toxin-derived peptides and proteins by heterologous expression is the strategy of choice for research groups and pharmaceutical industry to overcome this limitation. Recombinant expression in microorganisms is often the first choice, since bacteria and yeast systems combine high level of recombinant protein expression, fast cell growth and multiplication and simple media requirement. Herein, we present a comprehensive revision, which describes the scorpion venom components that were produced in their recombinant forms using microbial systems. In addition, we highlight the pros and cons of performing the heterologous expression of these compounds, regarding the particularities of each microorganism and how these processes can affect the application of these venom components. The most used microbial system in the heterologous expression of scorpion venom components is Escherichia coli (85%), and among all the recombinant venom components produced, 69% were neurotoxins. This review may light up future researchers in the choice of the best expression system to produce scorpion venom components of interest. (AU)

FAPESP's process: 16/04761-4 - Functional and structural characterization and immune response evaluation of a recombinant serine protease from Crotalus durissus collilineatus modified by PEGylation
Grantee:Ernesto Lopes Pinheiro Junior
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/16182-3 - New functional perspectives of a serine protease from Crotalus durissus collilineatus: activity on voltage-gated ion channels
Grantee:Johara Boldrini França Stringari
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/12317-3 - Cloning and heterologous expression of hyaluronidase and/or novel toxins obtained from the transcriptome of Tityus serrulatus' venom gland
Grantee:Fernanda Gobbi Amorim
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/14996-8 - Cloning and expression of animal toxins of biotechnological interest
Grantee:Eliane Candiani Arantes Braga
Support Opportunities: Regular Research Grants