Unveiling the fungal biotransformation of hydralazine using C-13-precursor

Author(s)

Almeida, Marilia O. ^[1] ; Lopes, Adriana A. ^[2] ; Roberto, Patricia G. ^[2] ; Bertoni, Bianca W. ^[2] ; Pupo, Monica T. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Sao Paulo - Brazil

^[2] Univ Ribeirao Preto, Unidade Biotecnol, Ave Constabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: PHYTOCHEMISTRY LETTERS; v. 26, p. 55-59, AUG 2018.
• Web of Science Citations: 1

Abstract

The fermentation of the endophyte Fusarium oxysporum SS46 and Fusarium oxysporum ATCC MYA 4623 with epigenetic modulator hydralazine in Czapek medium led to the isolation of 3-methyl-1,2,4-triazolo {[}3,4-alpha] phthalazine (1). The mechanism of biotransformation involves reaction with acetyl-CoA and further cyclization, and was unveiled using C-13-precursor. Additionally, AFLP technique for genomic DNA and differential gene expression (cDNA) analyses were applied for comparative profiling of the fungus under and without treatment with hydralazine. There was no change in the DNA profile of hydralazine-treated and non-treated cultures; however, cDNA-AFLP analyses showed a differential gene expression in the cultures after treatment of the fungi with hydralazine. (AU)

• FAPESP's process: 08/09540-0 - New strategies in the search of natural antibiotics: symbiotic microorganisms as sources of compounds and in vivo assay based on Caenorhabditis elegans infection model
• Grantee: Mônica Tallarico Pupo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC