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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Total Mercury Determination in Muscle and Liver Tissue Samples from Brazilian Amazon Fish Using Slurry Sampling

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de Queiroz, Joao Vitor [1] ; Souza Vieira, Jose Cavalcante [2] ; Bataglioli, Izabela da Cunha [1] ; Bittarello, Alis Correia [1] ; Braga, Camila Pereira [3] ; de Oliveira, Grasieli [1] ; Federici Padilha, Cilene do Carmo [2] ; Padilha, Pedro de Magalhes [2, 1]
Total Authors: 8
[1] So Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP - Brazil
[2] So Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 - USA
Total Affiliations: 3
Document type: Journal article
Source: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 184, n. 2, p. 517-522, AUG 2018.
Web of Science Citations: 7

This paper presents a slurry sampling method for total mercury determination by graphite furnace atomic absorption spectrometry (GFAAS) in tissue of fish from the Amazon. The tissue samples were lyophilized and macerated, and then the slurry samples were prepared by putting 20 mg of tissue, added to a solution containing Triton X-100, Suprapur HNO3, and zirconium nitrate directly in sampling vials of a spectrometer. Mercury standard solutions were prepared under the same conditions as the slurry samples. The slurry samples and the mercury standard solutions were sonicated for 20 s. Twenty microliters of slurry samples were injected into the graphite tube, which contained an internal wall lined with tungsten carbide. Under these conditions, it was possible to thermally stabilize the mercury up to an atomization temperature of 1700 A degrees C. The method was validated by mercury determination in reference materials DORM-4 and DOLT-4. The LOD and LOQ were 0.014 and 0.045 mg kg(-1), respectively, and recovery percentages in relation to the concentration values were certified in the order of 98%. (AU)

FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants
FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants