Gold-Catalyzed C-S Aryl-Group Transfer in Zinc Finger Proteins

Author(s)

de Paiva, Raphael E. F. ^{[1, 2]} ; Du, Zhifeng ^[2] ; Nakahata, Douglas H. ^[1] ; Lima, Frederico A. ^{[3, 4]} ; Corbi, Pedro P. ^[1] ; Farrell, Nicholas P. ^[2]

Affiliation

^[1] Univ Campinas UNICAMP, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[2] Virginia Commonwealth Univ, Dept Chem, 1001 W Main St, Richmond, VA 23284 - USA

^[3] European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld - Germany

^[4] Brazilian Synchrotron Light Lab LNLS, Ctr Nacl Pesquisa Energia & Mat, BR-13084971 Campinas, SP - Brazil

• Document type: Journal article
• Source: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION; v. 57, n. 30, p. 9305-9309, JUL 20 2018.
• Web of Science Citations: 8

Abstract

Reaction of the Au-CN chelate {[}Au(bnpy)Cl-2] with the full-length zinc finger (ZnF; ZnCys(3)His) of HIV nucleocapsid protein NCp7 results in C-S aryl transfer from the Au-III organometallic species to a cysteine of the ZnF. The reaction is general and occurs even for finger3 of the transcription factor Sp1, containing a ZnCys(2)His(2) coordination sphere. This reaction is the first demonstration of group transfer from a coordination compound to biologically important zinc fingers, and is especially noteworthy for the ZnCys(2)His(2) transcription factors. The work expands the corpus of organometallic species which can efficiently modify biomolecules through C-atom transfer. The electronic features of the gold compound leading to this unexpected reaction were explored by X-ray absorption spectroscopy. (AU)

• FAPESP's process: 15/25114-4 - Synthesis of New Ag(I), Cu(II), Pd(II) and Pt(II) complexes based on sulfonamides and derivatives: antibacterial activities and application prospects on skin critically colonized wounds.
• Grantee: Pedro Paulo Corbi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/20882-3 - Metal complexes of sulfamethoxydiazine and sulfadimethoxine: synthesis, characterization and applications as antimicrobial agents for topical use
• Grantee: Douglas Hideki Nakahata
• Support Opportunities: Scholarships in Brazil - Doctorate