| Full text | |
| Author(s): |
da Silva, Aldeliane M.
[1]
;
Sahoo, Prasana K.
[1]
;
Cavalli, Alessandro
[2]
;
de Souza, Alessandra A.
[3]
;
Bakkers, Erik P. A. M.
[2]
;
Cesar, Carlos L.
[4]
;
Janissen, Richard
[5]
;
Cotta, Monica A.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Appl Phys Dept, BR-13083859 Campinas, SP - Brazil
[2] Eindhoven Univ Technol, Appl Phys Dept, NL-5600 MB Eindhoven - Netherlands
[3] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[4] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Campinas, SP - Brazil
[5] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | SMALL METHODS; v. 2, n. 7 JUL 10 2018. |
| Web of Science Citations: | 4 |
| Abstract | |
The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above approximate to 25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined. (AU) | |
| FAPESP's process: | 13/02300-1 - Semiconductor nanowires: formation mechanisms and biosensing applications |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control |
| Grantee: | Alessandra Alves de Souza |
| Support Opportunities: | Research Projects - Thematic Grants |