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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces

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Author(s):
da Silva, Aldeliane M. [1] ; Sahoo, Prasana K. [1] ; Cavalli, Alessandro [2] ; de Souza, Alessandra A. [3] ; Bakkers, Erik P. A. M. [2] ; Cesar, Carlos L. [4] ; Janissen, Richard [5] ; Cotta, Monica A. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Appl Phys Dept, BR-13083859 Campinas, SP - Brazil
[2] Eindhoven Univ Technol, Appl Phys Dept, NL-5600 MB Eindhoven - Netherlands
[3] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[4] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Campinas, SP - Brazil
[5] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands
Total Affiliations: 5
Document type: Journal article
Source: SMALL METHODS; v. 2, n. 7 JUL 10 2018.
Web of Science Citations: 3
Abstract

The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above approximate to 25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined. (AU)

FAPESP's process: 13/02300-1 - Semiconductor nanowires: formation mechanisms and biosensing applications
Grantee:Mônica Alonso Cotta
Support type: Regular Research Grants
FAPESP's process: 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control
Grantee:Alessandra Alves de Souza
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms
Grantee:Mônica Alonso Cotta
Support type: Regular Research Grants