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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Daily melatonin administration improves osseointegration in pinealectomized rats

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Author(s):
Palin, Leticia Pitol [1] ; Braga Polo, Tarik Ocon [2] ; de Souza Batista, Fabio Roberto [2] ; Silva Gomes-Ferreira, Pedro Henrique [2] ; Garcia Junior, Idelmo Rangel [2] ; Rossi, Ana Claudia [3] ; Freire, Alexandre [3] ; Faverani, Leonardo Perez [2] ; Sumida, Doris Hissako [1] ; Okamoto, Roberta [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Paulista, Fac Odontol, Dept Ciencias Basicas, BR-16015050 Aracatuba, SP - Brazil
[2] Univ Estadual Paulista, Fac Odontol, Dept Cirurgia & Clin Integrada, Aracatuba, SP - Brazil
[3] Univ Estadual Campinas, Fac Odontol Piracicaba, Area Anat, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Applied Oral Science; v. 26, 2018.
Web of Science Citations: 4
Abstract

Abstract The hypothesis of this study was that the peri-implant bone healing of the group of pinealectomized rats would differ from the control group. The samples were subjected to immunohistochemical, microtomographic (total porosity and connectivity density), and fluorochrome (mineralized surface) analyses. Objectives The goal of this study was to investigate the cellular changes and bone remodeling dynamics along the bone/implant interface in pinealectomized rats. Material and Methods The total of 18 adult male rats (Rattus norvegicus albinus, Wistar) was divided into three groups (n=6): control (CO), pinealectomized without melatonin (PNX) and pinealectomized with melatonin (PNXm). All animals were submitted to the first surgery (pinealectomy), except the CO group. Thirty days after the pinealectomy without melatonin, the second surgery was conducted, in which all animals received an implant in each tibia (36 titanium implants with surface treatment were installed – Implalife® São Paulo, SP, Brazil). By gavage, the rats of the PNX group received the vehicle solution, and the procedure. Results Immunohistochemical analysis for runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OP) and osteocalcin (OC) showed that the bone repair process in the PNXm group was similar to that of the CO group, whereas the PNX group showed a delay. The microtomographic parameters of total porosity [Po(tot)] and bone surface (BS) showed no statistically significant differences, whereas for the connective density (Conn.Dn) a statistical difference was found between the CO and PNXm groups. Fluorochrome analysis of the active mineralized surface showed statistically significant difference between the CO and PNX and between the CO and PNXm groups. Conclusion The absence of the pineal gland impaired the bone repair process during osseointegration, however the daily melatonin replacement was able to restore this response. (AU)

FAPESP's process: 14/11920-6 - Evaluation of bone healing in bone/implant interface in pinealectomized rats: histologicc, histometric, immunohistochemical, biomechanical and microCT analysis
Grantee:Tárik Ocon Braga Polo
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/15396-0 - Histometric and immunohistochemical analysis of bone repair in bone / implant interface in pinealectomized rats
Grantee:Letícia Pitol Palin
Support Opportunities: Scholarships in Brazil - Scientific Initiation