Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraiba Valley (Sao Paulo, Brazil)

Author(s)

Fazio, Alejandra T. ^{[1, 2]} ; Lopez, Monica Mamian ^[1] ; Temperini, Marcia L. A. ^[1] ; de Faria, Dalva Lucia A. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Lab Espectroscopia Mol, Av Prof Lineu Prestes 748, BR-05508000 Butanta, SP - Brazil

^[2] UBA, CONICET, Lab Micol Expt, DBBE, FCEyN, InMiBo, Piso 4, Pab 2, Lab 9, Int Guiraldes 2620, Ciudad Univ, Buenos Aires, DF - Argentina

• Document type: Journal article
• Source: VIBRATIONAL SPECTROSCOPY; v. 97, p. 129-134, JUL 2018.
• Web of Science Citations: 1

Abstract

In this work, Surface Enhanced Raman Spectroscopy (SEES) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraiba Valley (or Sao Paulo Historical Valley), which are representative of the first phase of the Brazilian coffee cycle (1820-1880). Very few studies are reported in the literature where SEES-based techniques are used in fungi identification, most of them focused on clinical diagnosis. In the present investigation, pure colonies isolated from biofilms on earthen walls previously identified by classic taxonomy and molecular biology were selected. The genera were Trichoderma, Cladosporium, Aspergillus, Neurospora, Fusarium and Penicillium. The fungi were cultured on solid potato dextrose agar, extracted with ethyl acetate and the extracts were applied on dried Au nanoparticles. The SEES spectra exhibited bands in the 600-1800 cm(-1) region which are characteristic of each genus, except Penicillium, as revealed by PCA statistical analysis. This work reports the use of a facile to prepare SEES-active substrate in the identification of microbial communities on earthen architecture walls and is the first step of an investigation aiming at the fast identification of fungi species from biofilms formed on earthen architecture buildings without the need of isolating the pure cultures. (AU)

• FAPESP's process: 12/13119-3 - Vibrational spectroscopy in condensed phases
• Grantee: Mauro Carlos Costa Ribeiro
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/07841-3 - Intensity and spectral fluctuations study in a single-molecule SERS spectra
• Grantee: Monica Benicia Mamian Lopez
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/05356-5 - Chemical aspects of biodeterioration of cultural heritage mineral substrates: rock art and historical buildings
• Grantee: Alejandra Teresa Fazio
• Support Opportunities: Scholarships in Brazil - Post-Doctoral