Implications on the Pb bioaccumulation and metallothionein levels due to dietary and waterborne exposures: The Callinectes danae case

Author(s)

Bordon, Isabella C. ^[1] ; Emerenciano, Andrews Krupinski ^[2] ; Costa Melo, Julia Reali ^[2] ; Machado Cunha da Silva, Jose Roberto ^[2] ; Teixeira Favaro, Deborah Ines ^[3] ; Gusso-Choueri, Paloma Kachel ^[1] ; de Campos, Bruno Galvao ^[1] ; de Souza Abessa, Denis Moledo ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, NEPEA, Campus Litoral Paulista, BR-11330900 Sao Vicente, SP - Brazil

^[2] Univ Sao Paulo, ICB, Dept Biol Celular & Desenvolvimento, Ave Prof Lineu Prestes, 1524 Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[3] IPEN, Ctr Reator Pesquisa CRPq, Ave Prof Lineu Prestes 2242 Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 162, p. 415-422, OCT 30 2018.
• Web of Science Citations: 2

Abstract

This study aimed to assess the bioaccumulation of Pb and induction of metallothionein-like proteins (MT) in Callinectes danae through single and combined dietary and waterborne exposures. Male C. danae individuals were collected in the south area of the Cananeia-Iguape-Peruibe Protected Area (APA-CIP), in Sao Paulo State, Brazil. After an acclimatization period, exposure assays were performed during 7 and 14 days, at two Pb concentrations (0.5 e 2.0 mu g/g) in 4 treatments: 1) control; 2) contaminated water only; 3) contaminated food only; 4) contaminated water and food. The results indicate that C. danae is highly tolerant to Pb exposure at the evaluated concentrations. In gills, Pb bioaccumulation is more dependent of water efflux and time of exposure (higher Pb values). However, pathways act simultaneously in the induction of MT expression in this tissue. The decreases in Pb accumulation in the combined treatments and MT increases after 14 days in gills suggests that these proteins play a detoxification function in the presence of Pb. In hepatopancreas, depending on the pre-dominance of a certain pathway or combined pathways, accumulation occured at different times. For muscle tissue, bioaccumulation was observed due to contaminated water exposure, but not dietary exposure, probably because Pb concentrations were low. (AU)

• FAPESP's process: 14/01576-6 - Assessment of the effects of exposure to Pb through respiration and feeding in blue crabs Callinectes danae
• Grantee: Isabella Cristina da Costa Leal Bordon
• Support Opportunities: Scholarships in Brazil - Post-Doctoral