Effect of ethanol-dissolved rhodamine B marker on mechanical properties of non-simplified adhesives

Author(s)

Machado, Camila Moreira ^{[1, 2]} ; Bim Junior, Odair ^[2] ; Giacomini, Marina Ciccone ^[2] ; Zardin Graeff, Marcia Sirlene ^[3] ; Pimentel Garcia, Fernanda Cristina ^[4] ; Rios, Daniela ^[5] ; Honorio, Heitor Marques ^[5] ; Wang, Linda ^[2]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Alameda Octavio Pinheiro Brisolla 9-75, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Integrated Res Ctr, Bauru Sch Dent, Bauru, SP - Brazil

^[4] Univ Brasilia UnB, Sch Hlth Sci, Dept Dent, Brasilia, DF - Brazil

^[5] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Collect Hlth, Bauru, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 84, p. 145-150, AUG 2018.
• Web of Science Citations: 0

Abstract

Objectives: Rhodamine B (RB) is commonly used to evaluate dental polymers, including dental bonding systems (DBS). For reliability assessments, its effect should not only allow visualization of the dentin-polymer interface but also must not interfere with the bonding of the DBS to dentin as measured by the microtensile bond strength and hardness tests. Material and methods: Flat human dentin surfaces were prepared and randomly distributed (n = 10) into six groups: Adper Scotchbond Multi-Purpose (MP) or Clearfil SE Bond (SE) in concentrations of none/control, 0.02 or 0.1 mg/mL. These combinations were prepared through ethanol dissolution to improve their penetration into the dentin. All specimens were fabricated with Filtek Z250 (n = 10) and prepared for a microtensile bond test (mu TBS) (0.5 mm/min) after 7 days and 6 months. The failure modes were determined using a stereomicroscope (x 40). For the hardness test, flat human dentin blocks were prepared and treated as previously described (n = 6). The specimens were stored at 37 degrees C/48 h and were tested (Knoop indenter - 25 gF/10 s). Data were analyzed with two-way ANOVA and Tukey tests for multiple comparisons (alpha = 0.05). The effect of time was evaluated using the Student t-test. Results: For 7-day mu TBS, both the DBS and RB concentrations were significant factors (p < 0.01). After 6 months, only the RB concentration was significantly different. Adhesive failures were prevalent for all groups. Regarding hardness, the DBS differed only with the use of 0.10 mg/mL of RB. Conclusions: Ethanol-dissolved rhodamine B in concentrations of 0.02 and 0.10 mg/mL in non-simplified adhesives can affect the physical-mechanical properties of functional monomer-based systems rather more than those of BisGMA systems. (AU)

• FAPESP's process: 13/08902-3 - Analysis of the effect of addition of Rhodamine B marker on mechanical properties of no-simplified dentin bonding systems
• Grantee: Camila Moreira Machado
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/13160-3 - Effect of addition of fluorescent agent (Rodhamine B) to no-simplified dentin bonding agents: analisys of physical-mechanical properties
• Grantee: Linda Wang
• Support Opportunities: Regular Research Grants