Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory Response of the Lungs

Author(s)

Ochoa-Amaya, Julieta E. ^{[1, 2]} ; Queiroz-Hazarbassanov, Nicolle ^[1] ; Namazu, Lilian B. ^[1] ; Calefi, Atilio S. ^[1] ; Tobaruela, Carla N. ^[1] ; Margatho, Rafael ^[1] ; Palermo-Neto, Joao ^[1] ; Ligeiro de Oliveira, Ana P. ^[3] ; Felicio, Luciano F. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Vet, Dept Patol, Sao Paulo - Brazil

^[2] Univ los Llanos, Programa Med Vet & Zootecnia, Fac Ciencias Agr & Recursos Nat, Villavicencio - Colombia

^[3] Univ Nove Julho, Programa Posgrad Biofoton Aplicada Ciencias Saud, Sao Paulo - Brazil

• Document type: Journal article
• Source: NEUROIMMUNOMODULATION; v. 25, n. 1, p. 34-41, 2018.
• Web of Science Citations: 0

Abstract

Purpose: We have previously shown that domperidone-induced short-term hyperprolactinemia reduces the lung's allergic inflammatory response in an ovalbumin antigenic challenge model. Since purinergic receptor P2X7R activity leads to proinflammatory cytokine release and is possibly related to the pathogenesis of allergic respiratory conditions, the present study was designed to investigate a possible involvement of purinergic and prolactin receptors in this phenomenon. Methods: To induce hyperprolactinemia, domperidone was injected intraperitoneally in rats at a dose of 5.1 mg x kg(-1) per day for 5 days. P2X7 expression was evaluated by lung immunohistochemistry while prolactin receptor expression in bronchoalveolar lavage leukocytes was analyzed through flow cytometry. Results: Previous reports demonstrated that rats subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bron-choalveolar lavage, especially granulocytes. Here, it is revealed that hyperprolactinemia promotes an increased expression of prolactin receptors in granulocytes. Also, increased expression of purinergic P2X7R observed in allergic animals was significantly reduced by hyperprolactinemia. Conclusions: Both purinergic and prolactin receptor expression changes occur during the anti-asthmatic effect of hyperprolactinemia. (C) 2018 S. Karger AG, Basel (AU)

• FAPESP's process: 09/51886-3 - Neuroimmunomodulation: drugs, stress and cytokines on nervous, endocrine and immune systems relationships
• Grantee: João Palermo Neto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/22364-2 - Hyperprolactinemia and allergic inflammatory response of the lungs
• Grantee: Luciano Freitas Felicio
• Support Opportunities: Regular Research Grants