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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Demonstration of viability and fertility and development of a molecular tool to identify YY supermales in a fish with both genotypic and environmental sex determination

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Author(s):
Hattori, Ricardo Shohei [1] ; Tashiro, Seiya [2] ; Zhang, Yan [2] ; Kakuta, Naoya [2] ; Yokota, Masashi [2] ; Strussmann, Carlos Augusto [2] ; Yamamoto, Yoji [2]
Total Authors: 7
Affiliation:
[1] APTA SAA, Unidade Pesquisa Desenvolvimento Campos Do Jordao, Campos Do Jordao - Brazil
[2] Tokyo Univ & Marine Sci & Technol, Grad Sch Marine Sci & Technol, Tokyo - Japan
Total Affiliations: 2
Document type: Journal article
Source: ECOLOGY AND EVOLUTION; v. 8, n. 15, p. 7522-7528, AUG 2018.
Web of Science Citations: 4
Abstract

The pejerrey possesses a genotypic sex determination system driven by the amhy gene and yet shows marked temperature-dependent sex determination. Sex-reversed XY females have been found in a naturally breeding population established in Lake Kasumigaura, Japan. These females could mate with normal XY males and generate YY supermale individuals that, if viable and fertile, would sire only genotypic male offspring. This study was conducted to verify the viability, gender, and fertility of YY pejerrey and to develop a molecular method for their identification. Production of YY fish was attempted by crossing a thermally sex-reversed XY female and an XY male, and rearing the progeny until sexual maturation. To identify the presumable YY individuals, we first conducted a PCR analysis using amhy-specific primers to screen only amhy-positive (XY and YY) fish. This screening showed that 60.6% of the progeny was amhy-positive, which suggested the presence of YY fish. We then conducted a second screening by qPCR in order to identify the individuals with two amhy copies in their genome. This screening revealed 13 individuals, all males, with values twice higher than the other 30 amhy-positive fishes, suggesting they have a YY complement. This assumption as well as the viability, fertility, and supermale nature of these individuals was confirmed in progeny tests with XX females that yielded 100% amhy-positive offspring. These results demonstrate that qPCR can obviate progeny test as a means to identify the genotypic sex and therefore may be useful for the survey of all three possible genotypes in wild populations. (AU)

FAPESP's process: 09/15877-0 - Evolution of the Y chromosome-linked gene amh (anti-Müllerian hormone) in atherinopsids and its role on genotypic sex determination (GSD)
Grantee:Ricardo Shohei Hattori
Support Opportunities: Scholarships in Brazil - Post-Doctoral