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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Leucine-rich diet alters the H-1-NMR based metabolomic profile without changing the Walker-256 tumour mass in rats

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Author(s):
Viana, Lais Rosa [1] ; Canevarolo, Rafael [2] ; Perina Luiz, Anna Caroline [1] ; Soares, Raquel Frias [1] ; Lubaczeuski, Camila [1] ; de Mattos Zeri, Ana Carolina [2] ; Cintra Gomes-Marcondes, Maria Cristina [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, Lab Nutr & Canc, BR-13083862 Campinas, SP - Brazil
[2] Brazilian Biosci Natl Lab, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BMC CANCER; v. 16, OCT 3 2016.
Web of Science Citations: 8
Abstract

Background: Cachexia is one of the most important causes of cancer-related death. Supplementation with branched-chain amino acids, particularly leucine, has been used to minimise loss of muscle tissue, although few studies have examined the effect of this type of nutritional supplementation on the metabolism of the tumour-bearing host. Therefore, the present study evaluated whether a leucine-rich diet affects metabolomic derangements in serum and tumour tissues in tumour-bearing Walker-256 rats (providing an experimental model of cachexia). Methods: After 21 days feeding Wistar female rats a leucine-rich diet, distributed in L-leucine and LW-leucine Walker-256 tumour-bearing groups, we examined the metabolomic profile of serum and tumour tissue samples and compared them with samples from tumour-bearing rats fed a normal protein diet (C - control; W - tumour-bearing groups). We utilised H-1-NMR as a means to study the serum and tumour metabolomic profile, tumour proliferation and tumour protein synthesis pathway. Results: Among the 58 serum metabolites examined, we found that 12 were altered in the tumour-bearing group, reflecting an increase in activity of some metabolic pathways related to energy production, which diverted many nutrients toward tumour growth. Despite displaying increased tumour cell activity (i.e., higher Ki-67 and mTOR expression), there were no differences in tumour mass associated with changes in 23 metabolites (resulting from valine, leucine and isoleucine synthesis and degradation, and from the synthesis and degradation of ketone bodies) in the leucine-tumour group. This result suggests that the majority of nutrients were used for host maintenance. Conclusion: A leucine rich-diet, largely used to prevent skeletal muscle loss, did not affect Walker 256 tumour growth and led to metabolomic alterations that may partially explain the positive effects of leucine for the whole tumour-bearing host. (AU)

FAPESP's process: 12/06955-0 - Metabolomic and proteomic profile and cell proliferation and apoptosis in Walker tumour-bearing rats under pregnancy hormones and leucine-rich diet
Grantee:Laís Rosa Viana
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/16115-1 - Biochemical and molecular mechanisms of cachexia. effects of Walker factor and leucine supplementation on myotube cell culture
Grantee:Maria Cristina Cintra Gomes Marcondes
Support Opportunities: Regular Research Grants
FAPESP's process: 10/00209-9 - Nutritional supplementation and cancer cachexia. Biochemical and molecular studies
Grantee:Maria Cristina Cintra Gomes Marcondes
Support Opportunities: Regular Research Grants