Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation

Author(s):Show less -

Avila, Carla Cristi ^[1] ; Mule, Simon Ngao ^[1] ; Rosa-Fernandes, Livia ^{[1, 2]} ; Viner, Rosa ^[3] ; Barison, Maria Julia ^[1] ; Costa-Martins, Andre Guillherme ^[1] ; de Oliveira, Gilberto Santos ^[1] ; Geraldes Teixeira, Marta Maria ^[1] ; Farias Marinho, Claudio Romero ^[1] ; Silber, Ariel Mariano ^[1] ; Palmisano, Giuseppe ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Southern Denmark, Dept Biochem & Mol Biol, DK-5230 Odense - Denmark

^[3] Thermo Fisher Sci, San Jose, CA 95134 - USA

• Document type: Journal article
• Source: GENES; v. 9, n. 8 AUG 2018.
• Web of Science Citations: 1

Abstract

Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state. In particular, T. cruzi epimastigotes are the replicative forms that colonize the intestine of the Triatomine insect vector before entering the stationary phase that is crucial for differentiation into metacyclic trypomastigotes, which are the infective forms of mammalian hosts. The transition from proliferative exponential phase to quiescent stationary phase represents an important step that recapitulates the early molecular events of metacyclogenesis, opening new possibilities for understanding this process. In this study, we report a quantitative shotgun proteomic analysis of the T. cruzi epimastigote in the exponential and stationary growth phases. More than 3000 proteins were detected and quantified, highlighting the regulation of proteins involved in different subcellular compartments. Ribosomal proteins were upregulated in the exponential phase, supporting the higher replication rate of this growth phase. Autophagy-related proteins were upregulated in the stationary growth phase, indicating the onset of the metacyclogenesis process. Moreover, this study reports the regulation of N-terminally acetylated proteins during growth phase transitioning, adding a new layer of regulation to this process. Taken together, this study reports a proteome-wide rewiring during T. cruzi transit from the replicative exponential phase to the stationary growth phase, which is the preparatory phase for differentiation. (AU)

• FAPESP's process: 16/06034-2 - The biological role of amino acids and their metabolites in Trypanosoma cruzi
• Grantee: Ariel Mariano Silber
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications
• Grantee: Giuseppe Palmisano
• Support Opportunities: Research Grants - Young Investigators Grants