Human Adipose-Derived CD146(+) Stem Cells Increase Life Span of a Muscular Dystrophy Mouse Model More Efficiently than Mesenchymal Stromal Cells

Author(s)

Gomes, Juliana P. ^[1] ; Coatti, Giuliana C. ^[1] ; Valadares, Marcos C. ^[1] ; Assoni, Amanda F. ^[1] ; Pelatti, Mayra V. ^[1] ; Secco, Mariane ^[1] ; Zatz, Mayana ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biosci, Human Genome & Stem Cell Res Ctr, Rua Matao, 106 Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: DNA AND CELL BIOLOGY; v. 37, n. 9, p. 798-804, SEP 2018.
• Web of Science Citations: 0

Abstract

Duchenne muscular dystrophy is the most common and severe form of progressive muscular dystrophy. Previous results showed an increased survival in double knockout mice (dko) when treated with adipose-derived CD146(+) cells. In this study, we analyzed the effect of CD146(+) cells compared to mesenchymal stem/stromal cells (MSCs) derived from the same human adipose sample when injected in the dko mouse model without immunosuppression. Both CD146(+) cells and MSCs increased the survival of treated mice when compared to vehicle-injected mice, with a more prominent effect of CD146(+) cells than MSCs. Both CD146(+) cells and MSCs suppressed peripheral blood mononuclear cell proliferation, indicating immunomodulatory properties. Co-culture experiments showed that MSCs have a more inflammatory profile expression, and angiogenesis assay showed that CD146(+) cells can improve blood vessel formation. CD146(+) cells can extend survival of muscular dystrophy mice more efficiently than MSCs, possibly due to immunomodulatory and angiogenic properties. Further investigations focusing on exogenous CD146(+) cell role in vivo will improve cell therapy understanding and effectiveness. (AU)

• FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
• Grantee: Mayana Zatz
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC