The effect of ovariectomy and 2 antiresorptive therapeutic agents on bone response in rats: A 3-dimensional imaging analysis

Author(s)

Romualdo, Priscilla Coutinho ^[1] ; Fernandes Furlanetto Cunha, Nayane Barbosa ^[1] ; Leoni, Graziela Bianchi ^[2] ; Sousa-Neto, Manoel Damiao ^[2] ; Consolaro, Alberto ^[3] ; de Queiroz, Alexandra Mussolino ^[1] ; Bezerra da Silva, Raquel Assed ^[1] ; Bezerra da Silva, Lea Assed ^[1] ; Nelson-Filho, Paulo ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Bauru Dent Sch, Dept Oral Pathol, Bauru, SP - Brazil

• Document type: Journal article
• Source: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 126, n. 3, p. 218-225, SEP 2018.
• Web of Science Citations: 0

Abstract

Objective. The aim of this study was to evaluate bone mineral density (BMD) and microarchitecture in femurs and maxillary bones of ovariectomized (OVX) rats treated or not treated with alendronate (ALD) or odanacatib (ODN). Study Design. Twenty rats were divided into groups: SHAM, OVX, OVX/ALD, and OVX/ODN. After 12 weeks, the femurs and maxillae were removed and subjected to 3-dimensional analysis by micro-computed tomography. Results were analyzed with 1-way analysis of variance and Tukey's post hoc test (alpha = 0.05). Results. OVX decreased maxillary and femoral BMD and altered femoral microarchitecture (P <.05). The drugs increased BMD of both types of bones, but only ALD maintained the phenotype similar to the SHAM group. The action of ALD was limited to the femoral trabecular separation (Tb.Sp). OVX and the drugs had no effect on the microarchitecture of the maxilla (P >.05). Conclusions. ALD and ODN therapy increased BMD in both bones after ovariectomy. ALD was more successful than ODN in preserving the morphology of bone similar to the SHAM group. ALD maintained the phenotype for Tb.Sp in the femur, but ODN did not. In the maxillae, neither ovariectomy nor the 2 antiresorptive drugs had significant effects on microarchitecture. (AU)

• FAPESP's process: 14/13238-8 - Evaluation of periapical lesions induced in female osteoporotic rats subjected or not to treatment with bisphosphonate or cathepsin K inhibitor
• Grantee: Paulo Nelson Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/18231-9 - Evaluation of induced apical periodontitis in osteoporotic mice submitted or not to treatment with alendronate
• Grantee: Priscilla Coutinho Romualdo
• Support Opportunities: Scholarships in Brazil - Doctorate