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Evaluation of bone repair after the installation of functionalized implants with anabolic medication associated with systemic administration of antiresorptive in estrogen deficient rats and metabolic syndrome

Grant number: 19/09946-0
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal researcher:Roberta Okamoto
Grantee:Ana Cláudia Ervolino da Silva
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Studies show a relationship between osteoporosis and metabolic syndrome, both conditions being present in a large part of the population. However, more studies are necessary, so that this correlation is better characterized. Many therapies are currently available for the treatment of osteoporosis, however, they may present some problems regarding long-term efficacy and safety. Thus, the present project aims to investigate the action of the association of systemic medication (oral bisphosphonate) and teriparatide as local medication, functionalizing the implant surfaces. For the realization of this project, it will be divided into 2 stages. The first step is to determine the best protocol for the functionalization of implants with teriparatide, using the layer by layer technique. Also in this step, physical and in vitro tests (cell cultures) will be performed to evaluate the properties of the functionalized surface, as for the improvement in the osteogenic responses. The second step will be in-vivo experiments to evaluate the effect of this functionalized surface during Peri-implant repair. For this, 96 adult Wistar rats will be divided into 6 large groups: 1. SHAM group (n = 16), where the animals will be submitted to fictitious ovariectomy and balanced diet. 2. SM group (n=16), where the animals will receive cafeteria diet. 3. OVX group (n=16), in which the animals will undergo ovariectomy surgery without drug treatment. 4. OVX/SM group (n=16), where the animals will undergo ovariectomy and the cafeteria diet. 5. OVX/SM/RIS group (n=16), in which the animals will undergo ovariectomy surgery and a cafeteria diet associated with drug treatment with risedronate sodium. 6. OVX/RIS group (n=16), in which the animals will undergo ovariectomy and treatment with risedronate sodium. In each large group there will be two subgroups: A- conventional implants and B-implants functionalized with teriparatide. After 30 days of initiation of drug treatment, the animals will be submitted to the extraction of the upper first molars bilaterally. After 30 days, these same rats will receive the implants in the region where the exodontia were performed. Euthanasia of the animals will be performed 60 days after implant installation. The collected jaws will be randomly selected by means of software for sample randomization for biomechanical (counter-torque) and real-time PCR (OPG, RANKL, OPN, ALP, OCN, VEGF, Wnt3a). The contra-lateral jaws will be used to analyze calcified cuts. Initially, the samples will be evaluated by computerized microtomography for the morphometric evaluation of the bone formed around the installed implants. Later, these same samples will be submitted to laboratory processing for calcified sections, for later analysis by confocal microscopy (Peri-implant bone dynamics, BIC and AON). The quantitative data will be submitted to the homoscedasticity test to select the appropriate statistical test (parametric or non-parametric), with a significance level of 5%. (AU)

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