The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei

Author(s)

de Paula, Renato Graciano ^[1] ; Campos Antonieto, Amanda Cristina ^[1] ; Carraro, Claudia Batista ^[1] ; Borges Lopes, Douglas Christian ^[1] ; Persinoti, Gabriela Felix ^[2] ; Aguiar Peres, Nalu Teixeira ^[3] ; Martinez-Rossi, Nilce Maria ^[4] ; Silva-Rocha, Rafael ^[5] ; Silva, Roberto Nascimento ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Biochem & Immunol, Mol Biotechnol Lab, Ribeirao Preto, SP - Brazil

^[2] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil

^[3] Sergipe Fed Univ UFS, Ctr Biol Sci & Hlth CCBS, Aracaju, SE - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Cell & Mol Biol, Syst & Synthet Biol Lab, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, OCT 8 2018.
• Web of Science Citations: 2

Abstract

In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Delta tmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation. (AU)

• FAPESP's process: 17/04206-3 - Elucidation of cellular signaling pathways mediated by protein kinase A (PKA), MAPK and calcium during expression and secretion of cellulases by the fungus Trichoderma reesei
• Grantee: Roberto do Nascimento Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/23233-9 - Characterization of calcium-dependent signaling pathway in the secretion and cellulase expression by Trichoderma reesei (Hypocrea jecorina
• Grantee: Renato Graciano de Paula
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/23653-2 - Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation of in Trichoderma reesei
• Grantee: Roberto do Nascimento Silva
• Support Opportunities: Regular Research Grants