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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spider silk proteome provides insight into the structural characterization of Nephila clavipes flagelliform spidroin

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Author(s):
Aparecido dos Santos-Pinto, Jose Roberto [1] ; Arcuri, Helen Andrade [1] ; Esteves, Franciele Grego [1] ; Palma, Mario Sergio [1] ; Lubec, Gert [2]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, BR-13500 Rio Claro, SP - Brazil
[2] Paracelsus Med Univ, A-5020 Salzburg - Austria
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, OCT 2 2018.
Web of Science Citations: 2
Abstract

The capture spiral of web from N. clavipes spider consists of a single type of spidroin - the flagelliform silk protein, a natural material representing a combination of strength and high elasticity. Flagelliform spider silk is the most extensible silk fibre produced by orb weaver spiders and the structure of this remarkable material is still largely unknown. In the present study we used a proteomic approach to elucidate the complete sequence and the post-translational modifications of flagelliform silk proteins. The long sequence of flagelliform silk protein presents 45 hydroxylated proline residues, which may contribute to explain the mechanoelastic property of these fibres, since they are located in the GPGGX motif. The 3D-structure of the protein was modelled considering the three domains together, i.e., the N- and C-terminal non-repetitive domains, and the central repetitive domain. In the resulting molecular model there is a predominance of random structures in the solid fibres of the silk protein. The N-terminal domain is composed of three alpha-helices and the C-terminal domain is composed of one small helical section. Proteomic data reported herein may be relevant for the development of novel approaches for the synthetic or recombinant production of novel silk-based spider polymers. (AU)

FAPESP's process: 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 10/19051-6 - Structural analysis of silk proteins of Nephila clavipes spider web by a proteomic approach.
Grantee:José Roberto Aparecido dos Santos-Pinto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/26451-9 - Bioprospecting and Structural Analysis of the Silk Proteins of Arthropods by a Proteomics Approach Using nanoLC-ESI-CID/ETD System
Grantee:José Roberto Aparecido dos Santos-Pinto
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 15/14220-8 - Proteometabolomic characterization of the spider web components Nephila clavipes used in prey capture strategy
Grantee:Franciele Grego Esteves
Support Opportunities: Scholarships in Brazil - Master