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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laboratory extraction of microalgal lipids using sugarcane bagasse derived green solvents

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de Jesus, Sergio S. [1] ; Ferreira, Gabriela F. [1] ; Fregolente, V, Leonardo ; Maciel Filho, Rubens [2]
Total Authors: 4
[1] Univ Estadual Campinas, Sch Chem Engn, Lab Optimizat Design & Adv Control, PO 6066, BR-13083852 Campinas, SP - Brazil
[2] Fregolente, Leonardo, V, Univ Estadual Campinas, Sch Chem Engn, Lab Optimizat Design & Adv Control, PO 6066, BR-13083852 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 5

Three green solvents were tested for lipid extraction based on the Bligh and Dyer method. Lipids from the microalgae Chlorella pyrenoidosa were extracted through the tertiary systems 2-methyltetrahydrofuran:isoamylalcohol:water (1:3, 1:2, 1:1, 2:1, 3:1 v/v) and 2-methyltetrahydrofuran:ethanol:water (3:1 v/v). For comparative effect, extractions with chloroform:methanol:water (3:1, 2:1, 1:1, 1:2, 1:3 v/v), with binary systems 2-methyltetrahydrofuran:water, isoamyl alcohol:water, chloroform:water, n-hexane:water, and monophasic systems 2-methyltetrahydrofuran (2-MeTHF), isoamyl alcohol, chloroform and n-hexane were used. The Bligh and Dyer method using green solvents was highly efficient, although some undesirable components were extracted, especially when larger volumes of isoamyl alcohol were used. System 2-MeTHF:ethanol:water formed only a biphasic system when it was used in a 3:1 (v/v) proportion, obtaining 64% of fatty acids. The chloroform:methanol (1:2 v/v) system extracted 122.2 +/- 1.9 mg g(-1), in which 92.4% consisted of fatty acids. In systems using green solvents, the extractions with the system 2-MeTHF:isoamyl alcohol (2:1 v/v) achieved a 78% selectivity (99.6 +/- 7.6 mg g(-1) of fatty acids) and an 88.2% efficiency when compared with the reference system chloroform:methanol 1:2 (v/v).The fatty acids profile showed that extractions with 2-MeTHF:isoamyl alcohol had higher percentages of fatty acids with low degree of unsaturation, which is ideal for the production of better quality biodiesel. The results obtained suggest that 2-MeTHF:isoamyl alcohol (2:1 v/v) can replace the chloroform:methanol system currently used. M an industrial level, this system still requires some technical improvements related to the energy expenditure for solvent evaporation, and at an economic level, the high price of the 2-MeTHF makes the process impracticable compared with hexane. (AU)

FAPESP's process: 15/19609-0 - Use of green solvents derived from sugarcane in the extraction of lipids and application in the production of biodiesel and synthesis of 2-methyltetrahydrofuran through bagasse
Grantee:Rubens Maciel Filho
Support type: Regular Research Grants
FAPESP's process: 10/03764-3 - Design and construction of agitated photobioreactor airlift for lipids and carbohydrates production
Grantee:Sergio Santos de Jesus
Support type: Scholarships in Brazil - Post-Doctorate