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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

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Author(s):
Correa, Isadora M. O. [1] ; Pereira, Larissa Q. [1] ; Silva, Isabella G. O. [1] ; Altarugio, Rafaela [1] ; Smaniotto, Bruna D. [1] ; Silva, Tarcisio M. [1] ; Okamoto, Adriano S. [1] ; Andreatti Filho, Raphael L. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista Unesp, Fac Med Vet & Zootecnia, Dept Clin Vet, Lab Patol Aviaria, BR-18618970 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 38, n. 7, p. 1300-1306, JUL 2018.
Web of Science Citations: 0
Abstract

ABSTRACT: Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively. (AU)

FAPESP's process: 13/04365-3 - Immunomagnectic separation assay associated with bacteriophage for Salmonella enterica diagnosis in chicken
Grantee:Raphael Lucio Andreatti Filho
Support Opportunities: Regular Research Grants