Phenolic profile by HPLC-MS, biological potential, and nutritional value of a promising food: Monofloral bee pollen

Author(s)

Machado De-Melo, Adriane Alexandre ^[1] ; Estevinho, Leticia M. ^{[2, 3]} ; Moreira, Manuela M. ^[4] ; Delerue-Matos, Cristina ^[4] ; de Freitas, Alex da Silva ^[5] ; Barth, Ortrud Monika ^{[6, 7]} ; de Almeida-Muradian, Ligia Bicudo ^[1]

Affiliation

^[1] Univ Sao Paulo, Pharmaceut Sci Sch, Dept Food & Expt Nutr, BR-05580000 Sao Paulo - Brazil

^[2] Polytech Inst Braganca, Agr Coll Braganca, Campus Santa Apolonia E, P-5301855 Braganca - Portugal

^[3] Univ Minho, Ctr Mol & Environm Biol, Campus Gualtar, P-4710057 Braga - Portugal

^[4] Polytech Inst Porto, Super Inst Engn, REQUIMTE LAQV, P-4200072 Porto - Portugal

^[5] Fed Fluminense Univ, Lab Marine Geol, BR-24210340 Niteroi, RJ - Brazil

^[6] Univ Fed Rio de Janeiro, Inst Geosci, Dept Geol, Lab Palynol, BR-21949900 Rio De Janeiro - Brazil

^[7] Fiocruz MS, Oswaldo Cruz Inst, Lab Viral Morphol & Morphogenesis, BR-21040360 Rio De Janeiro - Brazil

• Document type: Journal article
• Source: JOURNAL OF FOOD BIOCHEMISTRY; v. 42, n. 5 OCT 2018.
• Web of Science Citations: 5

Abstract

Practical applications The objective of this study was to determine physicochemical and phenolic profiles as well the antioxidant and antimicrobial capacities of monofloral bee pollen samples from Brazil. Traditional methods were used. The phenolic profile was determined by high-performance liquid chromatography-mass spectrometry (HPLC-MS). The protein (10.6-33.9 g/100g), lipids (3.2-8.3 g/100g), ashes (2.6-3.8 g/100g), total phenolic (5.6-29.7 mg GAE/g), and total flavonoid (0.3-19.0 mg QE/g) values were variable, even between products with the same botanical origin. The minerals analyzed were found in amounts varying between 10 mg/kg and 9.1 g/kg. Each product presented specific color parameters. Myrcia bee pollen presented the greater antioxidant capacity, and Mimosa scabrella bee pollen from Santa Catarina state was the most efficient in inhibiting bacteria and yeasts. Among the compounds identified by HPLC-MS, flavonoid 3-O-glycosides predominated. The physicochemical and phenolic profiles of each sample were distinct, and there was no pattern between monofloral products of the same pollen type. This manuscript reports physicochemical parameters, the biological potential, and the phenolic profile from HPLC-MS of some unknown monofloral bee pollen produced in Brazil. In addition to the possibility of the identification of phenolic compounds using a reliable technique, the current study helps to clarify one of the main questions of apicultural product researchers: Would monofloral bee pollens of the same botanical origin have similar profiles? Besides this, these results can increase consumer interest in this product and thus have positive impacts on apiculture in Brazil. (AU)

• FAPESP's process: 13/23179-6 - Phenolic compounds, microbiological quality and antimicrobial capacity of dehydrated bee pollen
• Grantee: Adriane Alexandre Machado de Melo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/11746-8 - Physicochemical characterization, microbiological quality, biological properties and botanical origin of dehydrated bee pollen
• Grantee: Adriane Alexandre Machado de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate