| Full text | |
| Author(s): |
Wailemann, Rosangela A. M.
[1]
;
Terra, Leticia F.
[1]
;
Oliveira, Talita C.
[1]
;
Dos Santos, Ancely F.
[1]
;
Gomes, Vinicius M.
[1]
;
Labriola, Leticia
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, Bloco 9 Sup, Sala 976, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Molecular and Cellular Endocrinology; v. 477, p. 39-47, DEC 5 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
The success of islet transplantation has improved lately. Unfortunately, it is still compromised by cell loss. We have shown that prolactin (PRL) inhibits beta-cell apoptosis and up-regulates the antiapoptotic Heat Shock Protein B1 (HSPB1) in human islets. Since its function in pancreatic islets has not been studied, we explored the role of HSPB1 in PRL-induced beta-cell survival. The significant PRL-induced cytoprotection in control cells was abrogated in HSPB1 silenced cells, overexpression of HSPB1 recovered survival. PRL-mediated inhibition of cytokine-induced caspase activities and cytokine-induced decrease of BCL-2/BAX ratio was significantly reverted in knocked-down cells. Kinetics of HSPB1 and HSF1 expression were studied in primary cultures of murine and human pancreatic islets. These findings are highly relevant for the improvement of clinical islet transplantation success rate since our results demonstrated a key role for HSPB1 pointing it as a promising target for beta-cell cytoprotection through the up-regulation of an endogenous protective pathway. (AU) | |
| FAPESP's process: | 14/17974-0 - Unveiling the role of HSPB1 in prolactin-induced cytoprotection in pancreatic beta cells |
| Grantee: | Vinícius de Morais Gomes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/02745-6 - Unveiling the molecular mechanisms involved in Prolactin-induced cytoprotection in Pancreatic Beta Cells. |
| Grantee: | Rosangela Aparecida Wailemann Mansano |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/13914-0 - Autophagy in beta-cells and the cytoprotective roles of prolactin |
| Grantee: | Letícia Ferreira Terra |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/07029-4 - Unveiling the role of HSP25/27 in Prolactin-induced cytoprotection in pancreatic beta cells |
| Grantee: | Leticia Labriola |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/04676-7 - Photodynamic therapy to treat breast cancer: proof of concept pre-clinical studies |
| Grantee: | Ancély Ferreira dos Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/03618-6 - Unveiling HSPB1's role in prolactin-induced modulation of the unfolded protein response in type 1 Diabetes models |
| Grantee: | Leticia Labriola |
| Support Opportunities: | Regular Research Grants |