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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Heat shock protein B1 is required for the prolactin-induced cytoprotective effects on pancreatic islets

Full text
Author(s):
Wailemann, Rosangela A. M. [1] ; Terra, Leticia F. [1] ; Oliveira, Talita C. [1] ; Dos Santos, Ancely F. [1] ; Gomes, Vinicius M. [1] ; Labriola, Leticia [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, Bloco 9 Sup, Sala 976, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 477, p. 39-47, DEC 5 2018.
Web of Science Citations: 1
Abstract

The success of islet transplantation has improved lately. Unfortunately, it is still compromised by cell loss. We have shown that prolactin (PRL) inhibits beta-cell apoptosis and up-regulates the antiapoptotic Heat Shock Protein B1 (HSPB1) in human islets. Since its function in pancreatic islets has not been studied, we explored the role of HSPB1 in PRL-induced beta-cell survival. The significant PRL-induced cytoprotection in control cells was abrogated in HSPB1 silenced cells, overexpression of HSPB1 recovered survival. PRL-mediated inhibition of cytokine-induced caspase activities and cytokine-induced decrease of BCL-2/BAX ratio was significantly reverted in knocked-down cells. Kinetics of HSPB1 and HSF1 expression were studied in primary cultures of murine and human pancreatic islets. These findings are highly relevant for the improvement of clinical islet transplantation success rate since our results demonstrated a key role for HSPB1 pointing it as a promising target for beta-cell cytoprotection through the up-regulation of an endogenous protective pathway. (AU)

FAPESP's process: 14/17974-0 - Unveiling the role of HSPB1 in prolactin-induced cytoprotection in pancreatic beta cells
Grantee:Vinícius de Morais Gomes
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/03618-6 - Unveiling HSPB1's role in prolactin-induced modulation of the unfolded protein response in type 1 Diabetes models
Grantee:Leticia Labriola
Support type: Regular Research Grants
FAPESP's process: 16/04676-7 - Photodynamic therapy to treat breast cancer: proof of concept pre-clinical studies
Grantee:Ancély Ferreira dos Santos
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/07029-4 - Unveiling the role of HSP25/27 in Prolactin-induced cytoprotection in pancreatic beta cells
Grantee:Leticia Labriola
Support type: Regular Research Grants
FAPESP's process: 14/02745-6 - Unveiling the molecular mechanisms involved in prolactin-induced cytoprotection in pancreatic beta cells
Grantee:Rosangela Aparecida Wailemann Mansano
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/13914-0 - Autophagy in beta-cells and the cytoprotective roles of prolactin
Grantee:Letícia Ferreira Terra
Support type: Scholarships in Brazil - Post-Doctorate