Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study

Author(s):Show less -

Passanezi Sant'Ana, Adriana Campos ^[1] ; Damante, Carla Andreotti ^[1] ; Frias Martinez, Maria Alejandra ^[1] ; Medina Valdivia, Maria Alejandra ^[1] ; Hage Karam, Paula Stefinia ^[1] ; de Oliveira, Flavia Amadeu ^[2] ; de Oliveira, Rodrigo Cardoso ^[2] ; Gasparoto, Thais Helena ^[3] ; Campanelli, Ana Paula ^[3] ; Ragghianti Zangrando, Mariana Schutzer ^[1] ; Rubo de Rezende, Maria Lucia ^[1] ; Aguiar Greghi, Sebastiao Luiz ^[1] ; Passanezi, Euloir ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Bauru, Discipline Periodont, Dept Prosthodont & Periodont, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Discipline Biochem, Dept Oral Biol, Sch Dent Bauru, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Discipline Microbiol & Immunol, Dept Oral Biol, Sch Dent Bauru, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Periodontology; v. 89, n. 11, p. 1326-1333, NOV 2018.
• Web of Science Citations: 1

Abstract

Background: The granulation tissue present in surgically-created early healing sockets has been considered as a possible source of osteoprogenitor cells for periodontal regeneration, as demonstrated in animal studies. However, the in vitro osteogenic properties of tissue removed from human surgically-created early healing alveolar defects (SC-EHAD) remains to be established, being that the aim of this study. Methods: Surgical defects were created in the edentulous ridge of two systemically healthy adults. The healing tissue present in these defects was removed 21 days later for the establishment of primary culture. The in vitro characteristics of the cultured cells were determined by Armelin method, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, immunohistochemistry, alkaline phosphatase (ALP) activity, mineralization assay, and flow cytometry for detection of stem cells/osteoprogenitor cell markers. Results: Cells were able to adhere to the plastic and assumed spindle-shaped morphology at earlier passages, changing to a cuboidal one with increasing passages. Differences in the proliferation rate were observed with increasing passages, suggesting osteogenic differentiation. ALP and mineralization activities were detected in conventional and osteogenic medium. Fresh samples of SC-EHAD tissue exhibited CD34(-) and CD45(-) phenotypes. Cells at later passages (14th) exhibited CD34(-), CD45(-), CD105(-), CD166(-), and collagen type I+ phenotype. Conclusion: Tissue removed from SC-EHAD is a possible source of progenitor cells. (AU)

• FAPESP's process: 12/14044-7 - Effects of PDGF-BB on the rate of proliferation and on the adhesion of human cells derived from bone repair tissue to root fragments
• Grantee: Adriana Campos Passanezi Santana
• Support type: Regular Research Grants