Analysis of healing of surgically created bone defects in rat calvaria treated with bovine grafts (Bio-Oss) associated with low level laser: histological and histometric study

• Grant number: 10/10538-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2011
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Ana Lucia Pompeia Fraga de Almeida
• Grantee: Ana Lucia Pompeia Fraga de Almeida
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Luis Augusto Esper

Abstract

With the increase in the utilization of endosseous implants for the rehabilitation of partially or totally edentulous patients, there must be satisfactory quality and volume of soft tissue and bone. Thus, the clinicians often face situations in which implants must be placed in less favorable positions due to the local bone quantity. Therefore, techniques and materials have been developed to promote predictable bone regeneration and allow the placement of implants in ideal position and angulation, which will provide better and more predictable restorations. Objectives: to histologically evaluate the bone healing process in surgically created critical size defects in rat calvaria treated with Bio-Oss (BO), in isolation or irradiated with low level laser (LL). Material and methods: the study will be conducted on 40 male rats (Rattus norvegicus, albinus, Wistar), weighing 250 to 300 g (University of São Paulo - USP, from the Central Animal Laboratory of Bauru Dental School - SP). For the accomplishment of all experimental procedures, the rats will be anesthetized via an intramuscular injection of xylazine (0.02 ml/kg) and ketamine hydrochloride (0.4 ml/kg). After trichotomy and antisepsis of the dorsal part of the skull of each animal, a half-moon incision will be made on the calvaria for posterior displacement of a full thickness flap. A critical size defect (CSD) with 5-mm diameter will be created with a trephine bur in low speed, under abundant irrigation with sterile saline. The animals will be divided into four study groups: 1) Control Group (Control), 2) Group LL (Low level laser - Thera Lase DMC, São Carlos, São Paulo), 3) Group BO (Bio-Oss®), 4) Group BO + LL (Bio-Oss® + Low level laser - Thera Lase DMC, São Carlos, São Paulo). The animals will be killed at 30 days postoperatively. After decalcification, each specimen will be longitudinally divided into two blocks, exactly through the center of the original surgically created defect. The specimens will then be processed and embedded in paraffin. Longitudinal serial sections with 6-µm thickness will be achieved, initiating from the center of the original surgically created defect. The sections will be stained with hematoxylin and eosin (H.E.) for light microscopy and histomorphometric analysis. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICOLE ROSA DE FREITAS; LUÍSA BELLUCO GUERRINI; LUIS AUGUSTO ESPER; MICHYELE CRISTHIANE SBRANA; GISELE DA SILVA DALBEN; SIMONE SOARES; ANA LÚCIA POMPÉIA FRAGA DE ALMEIDA. Evaluation of photobiomodulation therapy associated with guided bone regeneration in critical size defects. In vivo study. Journal of Applied Oral Science, v. 26, 2018-05-07. (10/13170-3, 10/10538-0, 11/00779-2)

DE ALMEIDA, ANA L. P. F.; MEDEIROS, ISABELLA L.; CUNHA, MERCIA J. S.; SBRANA, MICHYELE C.; DE OLIVEIRA, PAULA G. F. P.; ESPER, LUIS A.. The effect of low-level laser on bone healing in critical size defects treated with or without autogenous bone graft: an experimental study in rat calvaria. Clinical Oral Implants Research, v. 25, n. 10, p. 1131-1136, OCT 2014. (11/00779-2, 10/10538-0)

CUNHA, MERCIA J. S.; ESPER, LUIS A.; SBRANA, MICHYELE C.; DE OLIVEIRA, PAULA G. F. P.; DO VALLE, ACCACIO L.; DE ALMEIDA, ANA LUCIA P. F.. Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts: In Vivo Study in Rat Calvaria. BIOMED RESEARCH INTERNATIONAL, v. 2014, p. 9-pg., 2014-01-01. (10/10538-0, 10/13170-3)

SBRANA, MICHYELE CRISTHIANE; ESPER, LUIS AUGUSTO; DE FREITAS, NICOLE ROSA; GUERRINI, LUISA BELLUCO; HERNANDES JOB, PEDRO HENRIQUE; POMPEIA FRAGA DE ALMEIDA, ANA LUCIA. Evaluation of Different Photobiomodulation Therapy Protocols as Adjuncts in the Healing of Bone Defects Grafted with Inorganic Bovine Bone. INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, v. 37, n. 6, p. 6-pg., 2022-11-01. (10/10538-0)

CUNHA, MERCIA J. S.; ESPER, LUIS A.; SBRANA, MICHYELE C.; DE OLIVEIRA, PAULA G. F. P.; DO VALLE, ACCACIO L.; DE ALMEIDA, ANA LUCIA P. F.. Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts: In Vivo Study in Rat Calvaria. BIOMED RESEARCH INTERNATIONAL, 2014. (10/13170-3, 10/10538-0)

MOREIRA, GUILHERME SANTOS; MACHADO ALVES, PATRICK HENRY; ESPER, LUIS AUGUSTO; SBRANA, MICHYELE CRISTHIANE; DALBEN, GISELE DA SILVA; NEPPELENBROEK, KARIN HERMANA; POMPEIA FRAGA DE ALMEIDA, ANA LUCIA. Effect of Low-Level Laser on the Healing of Bone Defects Filled with Autogenous Bone or Bioactive Glass: In Vivo Study. INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, v. 33, n. 1, p. 169-174, JAN-FEB 2018. (10/10538-0)