The purpose of this study is to histomorphometrically analyze the influence of Bone Marrow Aspirate (BMA), Low-Level Laser Therapy (LLLT) or their combination on bone healing in surgically created critical-size defects (CSD) in rat calvaria. 40 rats will be divided into 4 groups: Group C (control), Group BMA, Group LLLT and Group BMA/LLLT. A 5 mm diameter CSD will be created in the calvarium of each animal. In Group C, the defect will be filled by blood clot only. In Group BMA, the defect will be filled with BMA. In Group LLLT, the defect will receive laser irradiation (GaAIAs laser), will be filled with blood clot and then will be irradiated again. In Group BMA/LLLT, the defect will receive laser irradiation (GaAIAs laser), will be filled with BMA and then will be irradiated again. All animals will be euthanized at 30 days post-operatively. Histometric, using image analysis software, and histologic analyses will be performed. Amount of newly formed bone will be calculated as a percentage of the total area of the original defect. The data will be submitted to statistical analysis.
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