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Analysis of healing of surgically created bone defects in rat calvaria treated with autogenous bone graft associated with low level laser. Histological and histometric study.

Grant number: 11/00779-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2011
Effective date (End): December 31, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Ana Lucia Pompeia Fraga de Almeida
Grantee:Isabella Luzia Medeiros
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Bone loss due to previous inflammatory disease, trauma or congenital anatomic deformities and is a major therapeutic problems in periodontology and implantology. Therefore, techniques and materials have been developed to promote predictable bone regeneration which will provide better and more predictable results. Objectives: to histologically evaluate the bone healing process in surgically created critical size defects in rat calvaria treated with autogenous bone (AO), in isolation or irradiated with low level laser (LL). Material and methods: the study will be conducted on 40 male rats (Rattus norvegicus, albinus, Wistar), weighing 250 to 300 g (University of São Paulo - USP, from the Central Animal Laboratory of Bauru Dental School - SP). For the accomplishment of all experimental procedures, the rats will be anesthetized via an intramuscular injection of xylazine (0.02 ml/kg) and ketamine hydrochloride (0.4 ml/kg). After trichotomy and antisepsis of the dorsal part of the skull of each animal, a half-moon incision will be made on the calvaria for posterior displacement of a full thickness flap. A critical size defect (CSD) with 5-mm diameter will be created with a trephine bur in low speed, under abundant irrigation with sterile saline. The animals will be divided into four study groups: 1) Control Group (Control), 2) Group LL (Low level laser - Thera Lase DMC, São Carlos, São Paulo), 3) Group AO (autogenous bone), 4) Group AO + LL (autogenous bone + Low level laser - Thera Lase DMC, São Carlos, São Paulo). The animals will be killed at 30 days postoperatively. After decalcification, each specimen will be longitudinally divided into two blocks, exactly through the center of the original surgically created defect. The specimens will then be processed and embedded in paraffin. Longitudinal serial sections with 6-µm thickness will be achieved, initiating from the center of the original surgically created defect. The sections will be stained with hematoxylin and eosin (H.E.) for light microscopy and histomorphometric analysis

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE ALMEIDA, ANA L. P. F.; MEDEIROS, ISABELLA L.; CUNHA, MERCIA J. S.; SBRANA, MICHYELE C.; DE OLIVEIRA, PAULA G. F. P.; ESPER, LUIS A.. The effect of low-level laser on bone healing in critical size defects treated with or without autogenous bone graft: an experimental study in rat calvaria. Clinical Oral Implants Research, v. 25, n. 10, p. 1131-1136, . (10/10538-0, 11/00779-2)
NICOLE ROSA DE FREITAS; LUÍSA BELLUCO GUERRINI; LUIS AUGUSTO ESPER; MICHYELE CRISTHIANE SBRANA; GISELE DA SILVA DALBEN; SIMONE SOARES; ANA LÚCIA POMPÉIA FRAGA DE ALMEIDA. Evaluation of photobiomodulation therapy associated with guided bone regeneration in critical size defects. In vivo study. Journal of Applied Oral Science, v. 26, . (11/00779-2, 10/10538-0, 10/13170-3)

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