Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

Author(s)

de Oliveira, Isadora Sousa ^[1] ; Manzini, Rafaella Varzoni ^[1] ; Ferreira, Isabela Gobbo ^[1] ; Cardoso, Iara Aime ^[1] ; Figueiredo Bordon, Karla de Castro ^[1] ; Thomazela Machado, Ana Rita ^[2] ; Greggi Antunes, Lusania Maria ^[2] ; Rosa, Jose Cesar ^{[3, 4]} ; Arantes, Eliane Candiani ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Prot Chem Ctr, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Mol & Cell Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 24, OCT 20 2018.
• Web of Science Citations: 0

Abstract

Abstract Background: In recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. Disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ECM interactions. These molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogenesis. Therefore, disintegrin from Crotalus durissus collilineatus venom was isolated, structurally characterized and evaluated for its toxicity and ability to interfere with cell proliferation and migration in MDA-MB-231, a human breast cancer cell line. Methods: Based on previous studies, disintegrin was isolated by FPLC, through two chromatographic steps, both on reversed phase C-18 columns. The isolated disintegrin was structurally characterized by Tris-Tricine-SDS-PAGE, mass spectrometry and N-terminal sequencing. For the functional assays, MTT and wound-healing assays were performed in order to investigate cytotoxicity and effect on cell migration in vitro, respectively. Results: Disintegrin presented a molecular mass of 7287.4 Da and its amino acid sequence shared similarity with the disintegrin domain of P-II metalloproteases. Using functional assays, the disintegrin showed low cytotoxicity (15% and 17%, at 3 and 6 μg/mL, respectively) after 24 h of incubation and in the wound-healing assay, the disintegrin (3 μg/mL) was able to significantly inhibit cell migration (24%, p < 0.05), compared to negative control. Conclusion: Thus, our results demonstrate that non-RGD disintegrin from C. d. collilineatus induces low cytotoxicity and inhibits migration of human breast cancer cells. Therefore, it may be a very useful molecular tool for understanding ECM-cell interaction cancer-related mechanisms involved in an important integrin family that highlights molecular aspects of tumorigenesis. Also, non-RGD disintegrin has potential to serve as an agent in anticancer therapy or adjuvant component combined with other anticancer drugs. (AU)

• FAPESP's process: 16/11578-1 - Functional study of a disintegrin from Crotalus durissus collilineatus venom
• Grantee: Rafaella Varzoni Manzini
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/14996-8 - Cloning and expression of animal toxins of biotechnological interest
• Grantee: Eliane Candiani Arantes Braga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/03580-9 - Biochemical, structural and functional evaluation of a phosphodiesterase from Crotalus durissus collilineatus venom
• Grantee: Isadora Sousa de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate