Internal standard evaluation for bioimaging soybean leaves through laser ablation inductively coupled plasma mass spectrometry: a plant nanotechnology approach

Author(s)

Chacon-Madrid, Katherine ^{[1, 2]} ; Zezzi Arruda, Marco Aurelio ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Spectrometry Sample Preparat & Mech Grp, GEPAM, Inst Chem, Dept Analyt Chem, POB 6154, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Natl Inst Sci & Technol Bioanalyt, Dept Analyt Chem, INCTBio, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Analytical Atomic Spectrometry; v. 33, n. 10, p. 1720-1728, OCT 1 2018.
• Web of Science Citations: 2

Abstract

This work evaluates the use of an internal standard for acquiring the elemental mapping of Ag-107, Mn-55 and Cu-63 using laser ablation inductively coupled plasma mass spectrometry in the imaging mode from soybean leaves cultivated in the absence or in the presence of silver nanoparticles (40 nm average size) and also using silver nitrate for comparative purposes. Carbon, which is naturally present in plants and currently used as an internal standard for plant analysis is evaluated as C-12 and C-13, as well as Si-28 and P-31, as viable alternatives. Optimized conditions are established using a certified reference material - CRM 1573a Tomato leaves - considering those parameters involved in the plasma production (RF power - 1000 W and carrier and auxiliary gas flow rates - 1.1 L min(-1) and 1.8 L min(-1), respectively) as well as in the laser ablation process (laser intensity - 55%, frequency - 20 Hz, spot size diameter - 80 m, and scan speed - 70 m s(-1)). Additionally, the choice of the correct internal standard (C-13(+)) is made based on the highest precision obtained (RSD of 37, 15, and 4% for Ag-107(+), Mn-55(+), and Cu-63(+), respectively) as well as the highest homogeneity in the sample surface (verified through the images). The analysis of the images reveals not only the basal level of Ag present in the leaves, indicating its poor translocation, but also the homeostasis of Mn and Cu highly affected in plants cultivated in the presence of silver nitrate compared to those cultivated in the presence of silver nanoparticles. The distribution of Mn and Cu in the leaves is similar when the control plants and those cultivated in the presence of nanoparticles are compared. (AU)

• FAPESP's process: 16/07384-7 - Elementary bioimaging through laser ablation and inductively coupled plasma mass spectrometry
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Regular Research Grants