Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil

Author(s):Show less -

de Souza, William Marcie ^{[1, 2]} ; Fumagalli, Marcilio Jorge ^[1] ; Torres Carrasco, Adriano de Oliveira ^[3] ; Romeiro, Marilia Farignoli ^[1] ; Modha, Sejal ^[2] ; Seki, Meire Christina ^[3] ; Gheller, Janaina Menegazzo ^[3] ; Daffre, Sirlei ^[4] ; Teixeira Nunes, Marcio Roberto ^[5] ; Murcia, Pablo Ramiro ^[2] ; Acrani, Gustavo Olszanski ^[6] ; Moraes Figueiredo, Luiz Tadeu ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Glasgow, Ctr Virus Res, MRC, Glasgow G61 1QH, Lanark - Scotland

^[3] Univ Ctr Oeste Parana, BR-85015430 Guarapuava, PR - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Lab Bioquim & Imunol Artropode, BR-05508900 Sao Paulo, SP - Brazil

^[5] Inst Evandro Chagas, Ctr Technol Innovat, BR-67030000 Ananindeua, PA - Brazil

^[6] Univ Fed Fronteira Sul, BR-99010200 Passo Fundo, RS - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, NOV 5 2018.
• Web of Science Citations: 12

Abstract

Ticks are ectoparasites spread worldwide and are well known as vectors of many viruses of great importance to human and animal health. However, the viral diversity in ticks is still poorly understood, particularly in South America. Here we characterized the viral diversity present in Rhipicephalus microplus parasitizing cattle in the southern region of Brazil using metagenomics. Our study revealed the presence of viruses that had not been previously described in the region, including lihan tick virus (Phenuiviridae family) and wuhan tick virus 2 (Chuviridae family), as well as expands the biogeography of jingmen tick virus (Flaviviridae family) in Brazil. Also, we described three novel tymoviruses (Tymovirales order), named guarapuava tymovirus-like 1 to 3. We described the genomic and phylogenetic characterization of these viruses. Our study sheds light on the viral diversity of Rhipicephalus microplus in South America, and also expands the biogeography of tick viruses that were previously described only in Asia. (AU)

• FAPESP's process: 16/01414-1 - Production of recombinant viral antigenic peptides and development of methods for serological diagnosis of infections by Chikungunya, Mayaro and Zika viruses
• Grantee: Marcilio Jorge Fumagalli
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/02438-6 - Studies with Bunyaviridae that produce human disease
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/05778-5 - Bioinformatics analysis of data of viral metagenomic of animals from Brazil and Antarctica
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/14929-1 - Detection of arbovirus and other RNA viruses by real time RT-PCR and study of the viroma
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/13981-0 - Characterization, genomics and diagnostic of viruses with importance for public health in Brazil by high throughput sequencing
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
• Grantee: Marilia Farignoli Romeiro
• Support Opportunities: Scholarships in Brazil - Doctorate