| Full text | |
| Author(s): Show less - |
Cernak, Paul
[1]
;
Estrela, Raissa
[1]
;
Poddar, Snigdha
[1, 2]
;
Skerker, Jeffrey M.
[3, 4]
;
Cheng, Ya-Fang
[5]
;
Carlson, Annika K.
[3]
;
Chen, Berling
[6]
;
Glynn, Victoria M.
[7]
;
Furlan, Monique
[8]
;
Ryan, Owen W.
[1]
;
Donnelly, Marie K.
[9]
;
Arkin, Adam P.
[3, 4, 10]
;
Taylor, John W.
[9]
;
Cate, Jamie H. D.
[6, 1, 11]
Total Authors: 14
|
| Affiliation: Show less - | [1] Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 - USA
[2] Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 - USA
[3] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 - USA
[4] Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA - USA
[5] Energy Biosci Inst, Berkeley, CA - USA
[6] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 - USA
[7] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 - USA
[8] Univ Estadual Campinas, Dept Genet Evolut & Bioagents, Campinas, SP - Brazil
[9] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 - USA
[10] Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA - USA
[11] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging, Berkeley, CA 94720 - USA
Total Affiliations: 11
|
| Document type: | Journal article |
| Source: | MBIO; v. 9, n. 5 SEP-OCT 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available to probe its biology. However, S. cerevisiae has proven difficult to engineer to expand the carbon sources it can utilize, the products it can make, and the harsh conditions it can tolerate in industrial applications. Other yeasts that could solve many of these problems remain difficult to manipulate genetically. Here, we engineered the thermotolerant yeast Kluyveromyces marxianus to create a new synthetic biology platform. Using CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats with Cas9)-mediated genome editing, we show that wild isolates of K. marxianus can be made heterothallic for sexual crossing. By breeding two of these mating-type engineered K. marxianus strains, we combined three complex traits-thermotolerance, lipid production, and facile transformation with exogenous DNA-into a single host. The ability to cross K. marxianus strains with relative ease, together with CRISPR-Cas9 genome editing, should enable engineering of K. marxianus isolates with promising lipid production at temperatures far exceeding those of other fungi under development for industrial applications. These results establish K. marxianus as a synthetic biology platform comparable to S. cerevisiae, with naturally more robust traits that hold potential for the industrial production of renewable chemicals. IMPORTANCE The yeast Kluyveromyces marxianus grows at high temperatures and on a wide range of carbon sources, making it a promising host for industrial biotechnology to produce renewable chemicals from plant biomass feedstocks. However, major genetic engineering limitations have kept this yeast from replacing the commonly used yeast Saccharomyces cerevisiae in industrial applications. Here, we describe genetic tools for genome editing and breeding K. marxianus strains, which we use to create a new thermotolerant strain with promising fatty acid production. These results open the door to using K. marxianus as a versatile synthetic biology platform organism for industrial applications. (AU) | |
| FAPESP's process: | 17/24957-3 - Development of a fluorescence-based approach for the high-throughput isolation of Kluyveromyces marxianus recombinant haploids |
| Grantee: | Monique Furlan |
| Support Opportunities: | Scholarships abroad - Research Internship - Scientific Initiation |